Analytical and Testing Center of Yanbian University, Gongyuan 977, Yanji 133002, China.
Talanta. 2010 Sep 15;82(4):1225-9. doi: 10.1016/j.talanta.2010.06.039. Epub 2010 Aug 2.
Flow-injection analysis system (FIA system), which was based on Fe(II)-catalyzed oxidation of chromotropic acid with hydrogen peroxide, was developed for the determination of hydrogen peroxide. The chromotropic acid has a fluorescence measured at lambda(em)=440 nm (emission wavelength) with lambda(ex)=235 nm (excitation wavelength), and the fluorescence intensity at lambda(em)=440 nm quietly decreased in the presence of hydrogen peroxide and Fe(II), which was caused by Fe(II)-catalyzed oxidation of chromotropic acid with hydrogen peroxide. By measuring the difference of fluorescence intensity, hydrogen peroxide (1.0 x 10(-8)-1.0 x 10(-3) mol L(-1)) could be determined by the proposed FIA system, whose analytical throughput was 40 samples h(-1). The relative standard deviation (RSD) was 1.03% (n=10) for 4.0 x 10(-8) mol L(-1) hydrogen peroxide. The proposed FIA technique could be applied to the determination of hydrogen peroxide in rain water samples.
流动注射分析系统（FIA 系统），基于 Fe(II)催化过氧氢氧化变色酸，被开发用于测定过氧化氢。变色酸在 lambda(em)=440nm（发射波长）处有荧光，lambda(ex)=235nm（激发波长），在存在过氧化氢和 Fe(II)的情况下，lambda(em)=440nm 处的荧光强度安静地降低，这是由 Fe(II)催化过氧氢氧化变色酸引起的。通过测量荧光强度的差异，可以通过所提出的 FIA 系统测定过氧化氢（1.0 x 10(-8)-1.0 x 10(-3) mol L(-1)），其分析吞吐量为 40 个样品 h(-1)。对于 4.0 x 10(-8) mol L(-1)过氧化氢，相对标准偏差（RSD）为 1.03%（n=10）。所提出的 FIA 技术可应用于雨水样品中过氧化氢的测定。
