基于 Fe(II) 催化铬变酸荧光猝灭的流动注射法测定过氧化氢。

Flow-injection determination of hydrogen peroxide based on fluorescence quenching of chromotropic acid catalyzed with Fe(II).

机构信息

Analytical and Testing Center of Yanbian University, Gongyuan 977, Yanji 133002, China.

出版信息

Talanta. 2010 Sep 15;82(4):1225-9. doi: 10.1016/j.talanta.2010.06.039. Epub 2010 Aug 2.

DOI:10.1016/j.talanta.2010.06.039

PMID:20801322

Abstract

Flow-injection analysis system (FIA system), which was based on Fe(II)-catalyzed oxidation of chromotropic acid with hydrogen peroxide, was developed for the determination of hydrogen peroxide. The chromotropic acid has a fluorescence measured at lambda(em)=440 nm (emission wavelength) with lambda(ex)=235 nm (excitation wavelength), and the fluorescence intensity at lambda(em)=440 nm quietly decreased in the presence of hydrogen peroxide and Fe(II), which was caused by Fe(II)-catalyzed oxidation of chromotropic acid with hydrogen peroxide. By measuring the difference of fluorescence intensity, hydrogen peroxide (1.0 x 10(-8)-1.0 x 10(-3) mol L(-1)) could be determined by the proposed FIA system, whose analytical throughput was 40 samples h(-1). The relative standard deviation (RSD) was 1.03% (n=10) for 4.0 x 10(-8) mol L(-1) hydrogen peroxide. The proposed FIA technique could be applied to the determination of hydrogen peroxide in rain water samples.

摘要

流动注射分析系统（FIA 系统），基于 Fe(II)催化过氧氢氧化变色酸，被开发用于测定过氧化氢。变色酸在 lambda(em)=440nm（发射波长）处有荧光，lambda(ex)=235nm（激发波长），在存在过氧化氢和 Fe(II)的情况下，lambda(em)=440nm 处的荧光强度安静地降低，这是由 Fe(II)催化过氧氢氧化变色酸引起的。通过测量荧光强度的差异，可以通过所提出的 FIA 系统测定过氧化氢（1.0 x 10(-8)-1.0 x 10(-3) mol L(-1)），其分析吞吐量为 40 个样品 h(-1)。对于 4.0 x 10(-8) mol L(-1)过氧化氢，相对标准偏差（RSD）为 1.03%（n=10）。所提出的 FIA 技术可应用于雨水样品中过氧化氢的测定。