Department of Psychology, University of Gothenburg , Gothenburg , Sweden ; Network for Empowerment and Well-Being , Sweden.
Network for Empowerment and Well-Being , Sweden ; Institute of Neuroscience and Physiology, Centre for Ethics, Law and Mental Health (CELAM), University of Gothenburg , Gothenburg , Sweden.
PeerJ. 2014 Aug 26;2:e531. doi: 10.7717/peerj.531. eCollection 2014.
1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) induces permanent neurochemical and functional deficits. Following the administration of either two or four injections of the dopamine neurotoxin, MPTP, at a dose of 40 mg/kg, C57/BL6 mice were given access to running-wheels (30-min sessions, four times/week, Monday-Thursday) and treatment with the treated yeast, Milmed(®) (four times/week, Monday-Thursday), or simply running-wheel exercise by itself, over ten weeks. It was observed that the combination of physical exercise and Milmed(®) treatment, the MPTP + Exercise + Yeast (MC) group [MPTP + Exercise + Milmed(®) (MC)], restored spontaneous motor activity markedly by test day 10, restored completely subthreshold L-Dopa-induced activity, and dopamine concentration to 76% of control values, in the condition wherein two administrations of MPTP (2 × 40 mg/kg) were given prior to initiation of exercise and/or Milmed(®) treatment. Physical exercise by itself, MPTP + Exercise (MC) group, attenuated these deficits only partially. Administration of MPTP four times (i.e., 40 mg/kg, s.c., once weekly over four weeks for a total of 160 mg/kg, MPTP + Exercise + Yeast (MC) group [MPTP + Exercise + Milmed(®) (SC)] and MPTP + Exercise (SC), induced a lesioning effect that was far too severe for either exercise alone or the exercise + Milmed(®) combination to ameliorate. Nevertheless, these findings indicate a powerful effect of physical exercise reinforced by Milmed(®) treatment in restoring MPTP-induced deficits of motor function and dopamine neurochemistry in mice.
1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）可导致永久性的神经化学和功能缺陷。在给予 40mg/kg 剂量的多巴胺神经毒素 MPTP 两次或四次注射后，C57/BL6 小鼠可进行跑步轮运动（30 分钟/次，每周 4 次，周一至周四），并接受治疗酵母 Milmed(®)（每周 4 次，周一至周四）或单独进行跑步轮运动，持续 10 周。结果表明，运动与 Milmed(®)治疗相结合（MPTP+运动+酵母，MC 组）[MPTP+运动+Milmed(®)（MC）]，在第 10 天测试日明显恢复了自发运动活动，完全恢复了阈下 L-Dopa 诱导的活动，以及多巴胺浓度恢复到对照值的 76%，在运动和/或 Milmed(®)治疗开始前给予两次 MPTP（2×40mg/kg）的情况下。单独运动本身（MPTP+运动，MC 组）仅部分减轻了这些缺陷。四次给予 MPTP（即 40mg/kg，sc，每周一次，四周共 160mg/kg，MPTP+运动+酵母，MC 组[MPTP+运动+Milmed(®)（SC）]和 MPTP+运动，SC）诱导的损伤效应过于严重，以至于单独运动或运动+Milmed(®)联合治疗都无法改善。然而，这些发现表明，Milmed(®)治疗增强的运动对恢复 MPTP 诱导的运动功能和多巴胺神经化学损伤具有强大的作用。
