El Dayem Soha M Abd, Battah Ahmed A, El Bohy Abo El Magd, El Shehaby Amal, El Ghaffar Esmat Abd
J Pediatr Endocrinol Metab. 2015 Mar;28(3-4):265-73. doi: 10.1515/jpem-2014-0215.
Our objective was to evaluate the relationship of plasma level of chemerin and vaspin to early atherosclerotic changes and cardiac autonomic neuropathy (CAN) in adolescent type 1 diabetic patients.
The study included 62 type 1 diabetic patients and 30 healthy volunteers of the same age and sex. Blood samples were taken for assessment of chemerin, vaspin, asymmetric dimenthylarginine (ADMA), and oxidized low-density lipoprotein (OxLDL) by enzyme-linked immunosorbent assay (ELISA) technique. Also, blood samples were taken for analysis of glycosylated hemoglobin; lipid profiles and urine samples were taken for assessment of albumin/creatinine ratio. Twenty-four-hour holter [for assessment of time domain heart rate variability (HRV)] and carotid intima-media thickness (CIMT) were also done. The t-test or Mann-Whitney U-test for independent variables, Pearson's or Spearman's correlation, and stepwise multiple regression analysis were used.
The mean age of diabetic patients was 16.3±1.5 years, and mean duration of diabetes was 9.4±2.9 years. Chemerin, vaspin, OxLDL, and albumin/creatinine ratio were significantly higher, whereas ADMA was significantly lower than the controls. By stepwise multiple regression analysis, vaspin had a relation with a standard deviation difference RR (SDARR) and waist/height ratio. Conversely, chemerin had a relation with OxLDL. Albumin/creatinine ratio had a significant positive correlation with chemerin and OxLDL, and a negative correlation with ADMA.
Type 1 diabetic patients had impaired time domain HRV associated with increased CIMT. Vaspin had a significant relation to CAN, whereas chemerin, ADMA, and OxLDL had a significant correlation with albumin/creatinine ratio that reflects their role in renal affection.
我们的目的是评估青少年1型糖尿病患者血浆中chemerin和vaspin水平与早期动脉粥样硬化改变及心脏自主神经病变（CAN）之间的关系。
该研究纳入了62例1型糖尿病患者以及30名年龄和性别匹配的健康志愿者。采集血样，采用酶联免疫吸附测定（ELISA）技术评估chemerin、vaspin、不对称二甲基精氨酸（ADMA）和氧化型低密度脂蛋白（OxLDL）。同时，采集血样分析糖化血红蛋白；采集血脂谱和尿样评估白蛋白/肌酐比值。还进行了24小时动态心电图监测[用于评估时域心率变异性（HRV）]以及颈动脉内膜中层厚度（CIMT）测量。对自变量采用t检验或Mann-Whitney U检验、Pearson或Spearman相关性分析以及逐步多元回归分析。
糖尿病患者的平均年龄为16.3±1.5岁，糖尿病平均病程为9.4±2.9年。与对照组相比，chemerin、vaspin、OxLDL和白蛋白/肌酐比值显著升高，而ADMA显著降低。通过逐步多元回归分析，vaspin与标准差差值RR（SDARR）及腰高比有关。相反，chemerin与OxLDL有关。白蛋白/肌酐比值与chemerin和OxLDL呈显著正相关，与ADMA呈负相关。
1型糖尿病患者存在时域HRV受损并伴有CIMT增加。Vaspin与CAN显著相关，而chemerin、ADMA和OxLDL与反映其在肾脏病变中作用的白蛋白/肌酐比值显著相关。
