Yang Huirong, Xie Zhenzhen, Li Shuisheng, Wu Xichao, Peng Cheng, Zhang Yong, Lin Haoran
a State Key Laboratory of Biocontrol , Institute of Aquatic Economic Animals, and The Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University , Guangzhou , China .
b College of Animal Science, South China Agricultural University , Guangzhou , China , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1674-6. doi: 10.3109/19401736.2014.958723. Epub 2014 Sep 11.
We present the complete mitochondrial genome of Epinephelus coioides in this study. The mitochondrial genome is 16,458 bp in length, containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region. All of them are encoded on the heavy strand except ND6 and 8 tRNA genes on the light strand. The nucleotide compositions of the light strand are 28.88% of G, 28.75% of T, 26.58% of A and 15.80% of C. All the protein-coding genes share ATG initiation codon except ATP6 with CTG and COXI with GTG, and four types of inferred termination codons are T (ND2, COXII, ND3, ND4 and Cytb), TA (COXIII), TAA (ND1, ATP8, ND4L, ND5, ATP6 and COXI) and TAG (ND6). There are 10 intergenic spacers, 5 gene overlaps and no tandem repeat sequence. Gene arrangement and distribution are consistent with the typical vertebrates.
在本研究中,我们展示了斜带石斑鱼的完整线粒体基因组。线粒体基因组长度为16,458 bp,包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和一个控制区。除了轻链上的ND6和8个tRNA基因外,所有这些基因都在重链上编码。轻链的核苷酸组成分别为:G占28.88%,T占28.75%,A占26.58%,C占15.80%。除ATP6的起始密码子为CTG、COXI的起始密码子为GTG外,所有蛋白质编码基因的起始密码子均为ATG,推测的终止密码子有四种类型,分别为T(ND2、COXII、ND3、ND4和Cytb)、TA(COXIII)、TAA(ND1、ATP8、ND4L、ND5、ATP6和COXI)和TAG(ND6)。有10个基因间隔区、5个基因重叠区,无串联重复序列。基因排列和分布与典型脊椎动物一致。