Liu Li, Yang Huirong, Yang Zituo, Zhao Huihong, Sun Jijia, Xiao Shibin, Yang Xiankuan, Li Guifeng
a College of Animal Science, South China Agricultural University , Guangzhou , China .
b Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding , South China Agricultural University , Guangzhou , China , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1671-3. doi: 10.3109/19401736.2014.958722. Epub 2014 Sep 11.
We present the complete mitochondrial genome of Protonibea diacanthus in this study. The mitochondrial genome is 16,535 bp in length, containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region. All of them are encoded on the heavy strand except ND6 and 8 tRNA genes on the light strand. The nucleotide compositions of the light strand are 31.09% of G, 27.54% of T, 25.34% of A and 16.03% of C. All the protein-coding genes start with ATG initiation codon except ATP6 with GTG, and 5 types of inferred termination codons are AGA (COXI and COXII), T (ND3, ND4, Cytb), TA (ND2, COXIII and ATP6), TAA (ND1, ATP8 and ND4L) and TAG (DN5 and ND6). There are 14 intergenic spacers and 7 gene overlaps. The tandem repeat sequence is observed in 12S-rRNA, 16S-rRNA, tRNA(Trp), tRNA(Ala), COXIII, ND4L and ND4 genes. Gene arrangement and distribution are consistent with the typical vertebrates.
在本研究中，我们展示了双棘原黄姑鱼的完整线粒体基因组。线粒体基因组长度为16,535 bp，包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和一个控制区。除了轻链上的ND6和8个tRNA基因外，所有基因都在重链上编码。轻链的核苷酸组成分别为：鸟嘌呤（G）31.09%、胸腺嘧啶（T）27.54%、腺嘌呤（A）25.34%、胞嘧啶（C）16.03%。除ATP6以GTG作为起始密码子外，所有蛋白质编码基因均以ATG作为起始密码子，推测的终止密码子有5种类型，分别为AGA（细胞色素氧化酶亚基I和II，COXI和COXII）、T（ND3、ND4、细胞色素b，Cytb）、TA（ND2、细胞色素氧化酶亚基III和ATP6，COXIII和ATP6）、TAA（ND1、ATP8和ND4L）和TAG（DN5和ND6）。有14个基因间隔区和7个基因重叠区。在12S-rRNA、16S-rRNA、tRNA（色氨酸，Trp）、tRNA（丙氨酸，Ala）、COXIII、ND4L和ND4基因中观察到串联重复序列。基因排列和分布与典型脊椎动物一致。
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