非洲大蜗牛（玛瑙螺科：玛瑙螺属）的完整线粒体基因组
The complete mitochondrial genome of the giant African snail Achatina fulica (Mollusca: Achatinidae).
作者信息
Yang Huirong, Zhang Jia-En, Guo Jing, Deng Zhixin, Luo Hao, Luo Mingzhu, Zhao Benliang
机构信息
a College of Animal Science, South China Agricultural University , Guangzhou , P.R. China .
b Institute of Tropical and Subtropical Ecology, South China Agricultural University , Guangzhou , P.R. China , and.
出版信息
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1622-4. doi: 10.3109/19401736.2014.958698. Epub 2014 Sep 18.
We present the complete mitochondrial genome of the Achatina fulica in this study. The results show that the mitochondrial genome is 15,057 bp in length, which is comprised of 13 protein-coding genes, 2 rRNA genes, 21 tRNA genes. The nucleotide compositions of the light strand are 35.47% of A, 27.97% of T 19.46% of C, and 17.10% of G. Except the ND3, 7 tRNA, ATP6, ATP8, COX3 and 12S-rRNA on the light strand, the rest are encoded on the heavy strand. Five types of inferred initiation codons are ATA (ND1, ND5), GTG (ND6), ATG (COX3, COX2), ATT (ND4) and TTG (COX1, ND2, ND3, ND4L, ATP6, ATP8, Cytb), and 3 types of inferred termination codons are T (COX3, ND2), TAA (ND1, ND4L, ND5, ND6, ATP6), and TAG (ND3, ND4, COX1, COX2, Cytb, ATP8). There are 24 intergenic spacers and 6 gene overlaps. The tandem repeat sequence (total 52 bp) of (AATAATT)n is observed in 16S-rRNA. Gene arrangement and distribution are inconsistent with the typical vertebrates.
在本研究中，我们展示了非洲大蜗牛完整的线粒体基因组。结果表明，该线粒体基因组长度为15,057 bp，由13个蛋白质编码基因、2个rRNA基因和21个tRNA基因组成。轻链的核苷酸组成分别为：A占35.47%，T占27.97%，C占19.46%，G占17.10%。除了轻链上的ND3、7个tRNA、ATP6、ATP8、COX3和12S - rRNA外，其余基因在重链上编码。推测的起始密码子有5种类型，分别是ATA（ND1、ND5）、GTG（ND6）、ATG（COX3、COX2）、ATT（ND4）和TTG（COX1、ND2、ND3、ND4L、ATP6、ATP8、Cytb），推测的终止密码子有3种类型，分别是T（COX3、ND2）、TAA（ND1、ND4L、ND5、ND6、ATP6）和TAG（ND3、ND4、COX1、COX2、Cytb、ATP8）。有24个基因间隔区和6个基因重叠区。在16S - rRNA中观察到（AATAATT）n的串联重复序列（共52 bp）。基因排列和分布与典型脊椎动物不一致。
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