Zhang Huixian, Li Fengbo, Zhu Xinrong, Meng Zhiqi
a College of Chemistry and Life Sciences, Zhejiang Normal University , Jinhua , P.R. China .
b State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control , Sericulture Research Institute, Zhejiang Academy of Agricultural Sciences , Hangzhou , P.R. China , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1652-3. doi: 10.3109/19401736.2014.958713. Epub 2014 Sep 11.
The complete mitochondrial genome of Bombyx mori strain Baiyun (Lepidoptera: Bombycidae) was determined in this study. The genome was 15,629 bp long with 37 typical animal mitochondrial genes and 1 non-coding A + T-rich region. Its gene content and order were identical to those of other lepidopteran mitochondrial genomes. All protein-coding genes (PCGs) were initiated by ATN codons except for the COI gene, which began with CGA codon. Eleven PCGs stopped with termination codon TAA, whereas the COI and COII genes ended with single T. All the tRNA genes showed typical secondary cloverleaf structures. The 496 bp AT-rich region contains several features common to other lepidopterans, such as the motif ATAGA followed by an 18-bp poly-T stretch and two microsatellite-like (TA)8 and (AT)9 elements preceded by the ATTTA motif.
本研究测定了家蚕白云品系(鳞翅目:蚕蛾科)的完整线粒体基因组。该基因组长度为15,629 bp,包含37个典型的动物线粒体基因和1个非编码富含A+T区域。其基因内容和顺序与其他鳞翅目线粒体基因组相同。除COI基因起始密码子为CGA外,所有蛋白质编码基因(PCGs)均由ATN密码子起始。11个PCGs以终止密码子TAA终止,而COI和COII基因以单个T结尾。所有tRNA基因均呈现典型的二级三叶草结构。496 bp的富含A区域包含其他鳞翅目昆虫共有的几个特征,如基序ATAGA后接18 bp的多聚T序列,以及在ATTTA基序之前的两个微卫星样(TA)8和(AT)9元件。