Park Eun Hye, Song Byung Min, Yum Jung, Kim Ji An, Oh Seung Kyoo, Kim Hyun Soo, Cho Gil Jae, Seo Sang Heui
1 Laboratory of Influenza Research, Chungnam National University , Daejeon, Republic of Korea.
Viral Immunol. 2014 Nov;27(9):449-62. doi: 10.1089/vim.2014.0029. Epub 2014 Sep 11.
Outbreaks of the highly pathogenic H5N1 virus in poultry and humans are ongoing. Vaccination is an efficient method for prevention of H5N1 infection. Using chickens and ducks, we assessed the efficacy of a vaccine comprising H5N1 hemagglutinin (HA) protein produced in a baculovirus expression system. The immunized chickens and ducks were protected against lethal infection by H5N1 in an antigen dose-dependent manner. Complete protection against homologous challenge and partial protection against heterologous challenge were achieved in chickens immunized with 5 μg HA protein and in ducks immunized with 10 μg HA protein. The IgG antibody subtype was mainly detected in the sera and tissues, including the lungs. The neuraminidase (NA) inhibition assay was negative in immunized chickens and ducks. Our results indicated that the expressed HA protein by baculovirus was immunogenic to both chickens and ducks, and the immunized chickens and ducks were protected from the lethal infections of highly pathogenic H5N1 influenza virus, though ducks required more HA protein than chickens to be protected. Also, baculovirus HA-vaccinated poultry can be differentiated from infected poultry by NA inhibition assay.
高致病性H5N1病毒在家禽和人类中的爆发仍在持续。疫苗接种是预防H5N1感染的有效方法。我们使用鸡和鸭评估了一种由杆状病毒表达系统产生的包含H5N1血凝素（HA）蛋白的疫苗的效力。免疫的鸡和鸭以抗原剂量依赖的方式免受H5N1的致死性感染。用5μg HA蛋白免疫的鸡和用10μg HA蛋白免疫的鸭分别实现了对同源攻击的完全保护和对异源攻击的部分保护。IgG抗体亚型主要在血清和包括肺在内的组织中检测到。免疫的鸡和鸭的神经氨酸酶（NA）抑制试验呈阴性。我们的结果表明，杆状病毒表达的HA蛋白对鸡和鸭均具有免疫原性，免疫的鸡和鸭可免受高致病性H5N1流感病毒的致死性感染，不过鸭比鸡需要更多的HA蛋白才能得到保护。此外，通过NA抑制试验可将杆状病毒HA疫苗接种的家禽与受感染的家禽区分开来。
