Agronomy Department, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia.
Institute of Agriculture and Tourism Poreč, K. Hugues 8, p.p. 31, 52 440 Poreč, Croatia.
Food Chem. 2011 Dec 1;129(3):1164-8. doi: 10.1016/j.foodchem.2011.05.097. Epub 2011 May 27.
Major chloroplast pigments in five leafy vegetables (chicory-Cichorium intybus, cv. 'Anivip' and cv. 'Monivip', dandelion-Taraxacum officinale, garden rocket-Eruca sativa and wild rocket-Diplotaxis tenuifolia), commonly consumed in Mediterranean countries, have been separated by high-performance liquid chromatography (HPLC) on a reversed-phase column. Three classes of pigments were identified and quantified: xanthophylls (oxygenated carotenoids), carotenes (hydrocarbon carotenoids) and chlorophylls. The contents of the pigments in the analysed leafy vegetables varied significantly. The results indicated that selected leafy vegetables were moderately rich in xanthophylls, primarily lutein (3.87-7.44mg/100g fwt). Other xanthophylls were detected in relatively small quantities. The provitamin A carotenoids (α- and β-carotene) were also detected, but α-carotene were not present in chicory cultivars and in dandelion. The ratio of chlorophyll a/b varied from 2.44 to 2.67 depending on the species. The highest content of all the analysed constituents was found in the garden rocket.
五种绿叶蔬菜（菊苣-菊苣 cv. 'Anivip' 和 cv. 'Monivip'、蒲公英-蒲公英 officinale、芝麻菜-芝麻菜 sativa 和野生火箭菜-芝麻菜 tenuifolia）中的主要叶绿体色素已通过高效液相色谱法（HPLC）在反相柱上分离。鉴定并定量了三类色素：叶黄素（含氧类胡萝卜素）、类胡萝卜素（烃类类胡萝卜素）和叶绿素。分析的绿叶蔬菜中的色素含量差异很大。结果表明，所选的绿叶蔬菜中叶黄素含量适中，主要是叶黄素（3.87-7.44mg/100g fwt）。其他叶黄素的含量相对较少。还检测到了维生素 A 前体类胡萝卜素（α-和β-胡萝卜素），但在菊苣品种和蒲公英中未检测到α-胡萝卜素。叶绿素 a/b 的比值因物种而异，范围为 2.44 至 2.67。在所分析的所有成分中，含量最高的是芝麻菜。
