Finkelman F D, Holmes J, Urban J F, Paul W E, Katona I M
Department of Medicine, Uniformed Services, University of the Health Sciences, Bethesda, MD 20814.
J Immunol. 1989 Jan 15;142(2):403-8.
To further define requirements for T cell help in the stimulation of an in vivo IgE response, we studied a system in which the injection of mice with a goat antibody to mouse IgD (GaMD) stimulates large polyclonal IgG1 and IgE responses. In this system, both responses are blocked by anti-CD4 antibody, but only the IgE response is blocked by (anti-IL-4) antibody. Anti-CD4 antibody, if injected 5 days after GaMD, was found to inhibit the GaMD-induced IgE response to a much greater extent than the IgG1 response, even though both responses occur simultaneously and are inhibited to an equal extent by optimal or suboptimal doses of anti-CD4 antibody administered 2 days after GaMD. Even a suboptimal, 50-micrograms dose of anti-CD4 antibody, when injected 5 days after GaMD, inhibited the IgE response to a much greater extent than did an optimal 10-mg dose of anti-IL-4 antibody injected at the same time, even though 10 mg of anti-IL-4 antibody more completely inhibited GaMD-induced IgE production than did 50 micrograms of anti-CD4 antibody when injected 2 days after GaMD. These observations provide evidence that a late acting form of T cell help other than IL-4 is important for the generation of an IgE response but not an IgG1 response in GaMD-immunized mice.
为了进一步明确在体内刺激IgE反应时T细胞辅助的需求，我们研究了一个系统，在该系统中给小鼠注射抗小鼠IgD山羊抗体（GaMD）可刺激大量多克隆IgG1和IgE反应。在这个系统中，两种反应均被抗CD4抗体阻断，但只有IgE反应被（抗IL-4）抗体阻断。发现如果在注射GaMD后5天注射抗CD4抗体，其对GaMD诱导的IgE反应的抑制程度远大于对IgG1反应的抑制程度，尽管两种反应同时发生，且在注射GaMD后2天给予最佳或次优剂量的抗CD4抗体时，它们受到的抑制程度相同。即使是次优剂量50微克的抗CD4抗体，在注射GaMD后5天注射时，对IgE反应的抑制程度也远大于同时注射的最佳剂量10毫克抗IL-4抗体，尽管在注射GaMD后2天注射时，10毫克抗IL-4抗体比50微克抗CD4抗体更能完全抑制GaMD诱导的IgE产生。这些观察结果提供了证据，表明除IL-4之外的一种晚期作用形式的T细胞辅助对于在GaMD免疫的小鼠中产生IgE反应而非IgG1反应很重要。
