Katona I M, Urban J F, Finkelman F D
Department of Pediatrics, F. Edward Herbert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD 20814.
J Immunol. 1988 May 1;140(9):3206-11.
The role of L3T4+ and Lyt-2+ T cells in protective immunity to Nippostrongylus brasiliensis (Nb) was studied in BALB/c mice that were depleted of either the L3T4+ or Lyt-2+ T cell population by injection with rat mAb specific for the appropriate determinant. Host responses to Nb infection including spontaneous elimination of adult worms, development of intestinal mucosal mast cell hyperplasia and the generation of a polyclonal IgE response were all completely blocked by 0.5 mg anti-L3T4 antibody administered simultaneously with Nb inoculation. However, administration of 0.5 mg of anti-Lyt-2 antibody at the same time and 7 days after inoculation with Nb had no effect on any of these responses. Injection of anti-L3T4 antibody as late as 9 days after Nb inoculation interfered with spontaneous cure of Nb infection and anti-L3T4 antibody injection 11 days after Nb inoculation inhibited serum IgE levels measured on day 13 by 50%. In addition, administration of anti-L3T4 antibody at the time of the peak serum IgE response, 13 days after Nb inoculation, accelerated the decline in serum IgE levels. Injection of previously Nb-infected mice with anti-L3T4 antibody at the time of a second Nb inoculation prevented the development of a secondary IgE response but did not affect immunity to Nb infection based on finding no adult worms in the intestines of these mice. These data indicate that 1) L3T4+ T cells are required for spontaneous cure of Nb infection, development of intestinal mucosal mast cell hyperplasia, and the generation and persistence of an IgE response during primary infection with Nb and 2) L3T4+ T cells are required for a considerable time after inoculation for optimal development of these responses. However, L3T4+ T cells are not required for all protective responses in immune mice. In contrast, our data indicate that considerable depletion of the Lyt-2+ T cell population has no significant effect on either worm expulsion or the generation of serum IgE responses.
在BALB/c小鼠中研究了L3T4⁺和Lyt-2⁺ T细胞对巴西日圆线虫（Nb）的保护性免疫作用。通过注射针对相应决定簇的大鼠单克隆抗体，使L3T4⁺或Lyt-2⁺ T细胞群体耗竭。与Nb接种同时给予0.5mg抗L3T4抗体，宿主对Nb感染的反应，包括成虫的自发清除、肠道黏膜肥大细胞增生的发展以及多克隆IgE反应的产生，均被完全阻断。然而，在接种Nb的同时及接种后7天给予0.5mg抗Lyt-2抗体，对这些反应均无影响。在接种Nb后9天注射抗L3T4抗体干扰了Nb感染的自发治愈，在接种Nb后11天注射抗L3T4抗体使第13天测得的血清IgE水平降低了50%。此外，在接种Nb后13天血清IgE反应达到峰值时给予抗L3T4抗体，加速了血清IgE水平的下降。在第二次接种Nb时给先前感染过Nb的小鼠注射抗L3T4抗体，可阻止二次IgE反应的发生，但基于在这些小鼠肠道中未发现成虫，表明其不影响对Nb感染的免疫力。这些数据表明：1）L3T4⁺ T细胞是Nb感染自发治愈、肠道黏膜肥大细胞增生发展以及初次感染Nb期间IgE反应的产生和持续所必需的；2）接种后相当长一段时间内，L3T4⁺ T细胞是这些反应最佳发展所必需的。然而，L3T4⁺ T细胞并非免疫小鼠所有保护性反应所必需。相比之下，我们的数据表明，Lyt-2⁺ T细胞群体的大量耗竭对蠕虫排出或血清IgE反应的产生均无显著影响。
