Brown K D, Zurawski S M, Mosmann T R, Zurawski G
Department of Molecular Biology, DNAX Research Institute, Palo Alto, CA 94304.
J Immunol. 1989 Jan 15;142(2):679-87.
We have isolated and characterized four cDNA clones that encode mRNA expressed more abundantly in Con A-activated mouse helper T cells than by resting T cells. One mRNA encoded a approximately 14-kDa protein with a hydrophobic N-terminal sequence and was abundantly expressed by the Th 2 subset of Th cells, but was not expressed by Th 1 cells. The remaining three mRNA encoded related approximately 8-kDa secreted proteins that are part of a family of small, secreted, and inducible mouse and human proteins. This family of proteins is itself distantly related to another family of growth and inflammatory factors that are associated with various lymphoid and fibroblast activation phenomena. One of the small, inducible, secreted proteins has a predicted mature N terminus identical to that of the previously described macrophage inflammatory protein.
我们已经分离并鉴定了四个cDNA克隆，它们编码的mRNA在伴刀豆球蛋白A激活的小鼠辅助性T细胞中比在静止T细胞中表达更为丰富。一种mRNA编码一种约14 kDa的蛋白质，其N端序列具有疏水性，在Th细胞的Th 2亚群中大量表达，但在Th 1细胞中不表达。其余三种mRNA编码相关的约8 kDa分泌蛋白，它们是一个小型、可分泌且可诱导的小鼠和人类蛋白质家族的一部分。该蛋白质家族本身与另一个与各种淋巴细胞和成纤维细胞激活现象相关的生长和炎症因子家族关系较远。其中一种小型、可诱导、分泌的蛋白质预测的成熟N端与先前描述的巨噬细胞炎症蛋白相同。
