Office of Academic Affairs, Shandong Agricultural University, Tai'an 271018, China; State Key Laboratory of Crop Biology, Tai'an 271018, Shandong, China.
State Key Laboratory of Crop Biology, Tai'an 271018, Shandong, China; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, Shandong, China.
Food Chem. 2011 Jun 15;126(4):1959-63. doi: 10.1016/j.foodchem.2010.12.052. Epub 2010 Dec 15.
A novel method for purifying gingerols from ginger was developed using a high-speed counter-current chromatography (HSCCC). The two-phase solvent system such as light petroleum (bp 60-90°C)-ethyl acetate-methanol-water (5:5:6.5:3.5, v/v/v/v) was applied to the separation and purification of 6-, 8- and 10-gingerol from a crude extract of ginger. The experiment yielded 30.2mg of 6-gingerol, 40.5mg of 8-gingerol, 50.5mg of 10-gingerol from 200mg of crude extract in one-step separation. And the purity of these compounds was 99.9%, 99.9% and 99.2%, respectively, as determined by high-performance liquid chromatography (HPLC). Their structures were identified by gas chromatography-mass spectrometry (GC/MS) and (1)H, (13)C nuclear magnetic resonance (NMR).
一种从生姜中提取姜烯酚的新方法是利用高速逆流色谱（HSCCC）开发的。采用轻石油（bp 60-90°C）-乙酸乙酯-甲醇-水（5:5:6.5:3.5，v/v/v/v）两相溶剂系统，从生姜粗提取物中分离和纯化 6-、8-和 10-姜烯酚。实验从 200mg 的粗提取物中一步分离得到 30.2mg 的 6-姜烯酚、40.5mg 的 8-姜烯酚和 50.5mg 的 10-姜烯酚。这些化合物的纯度分别通过高效液相色谱（HPLC）测定为 99.9%、99.9%和 99.2%。通过气相色谱-质谱（GC/MS）和（1）H、（13）C 核磁共振（NMR）鉴定了它们的结构。
