ICU, Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China (Chen L); General Internal Department, First Affiliated Hospital, Sun Yat-sen University (Tang H, Liang YB, Chen ZB, Li ZY, Ma ZF); Emergency Medicine Department, Second Affiliated Hospital, Sun Yat-sen University (Huang ZT, Jiang LY).
World J Emerg Med. 2010;1(2):99-103.
As the regulators of cytokines, suppressors of cytokine signaling (SOCS) play an important role in the inflammation reaction. Some studies found that SOCS-1 and SOCS-3 were involved in the pathogenesis of some inflammatory diseases such as rheumatoid arthritis, inflammatory bowel disease. But the expressions of SOCS in coronary heart disease have not yet been reported. This study aimed to investigate the expression and clinical significance of SOCS-1 and SOCS-3 in the myocardium of patients with sudden cardiac death (SCD).
Myocardial autopsy specimens were collected from 24 patients at the Forensic Medicine Department of Sun Yat-Sen University, Guangzhou, China between 2005 and 2006. Of them, 9 patients had autopsy findings consistent with coronary atherosclerosis (non-myocardial infarction) leading to SCD (non-MI group), 7 died of acute myocardial infaction (MI group), and 8 died from traffic accidents and trauma (control group). The expressions of SOCS-1 mRNA and SOCS-3 mRNA in the myocardium of the non-MI, MI and control groups were detected using RT-PCR. The levels of SOCS-1 and SOCS-3 proteins were detected using immunohistochemistry. Statistical analyses were performed using SPSS version 13.0 software and the data were analyzed by ANOVA.
The expressions of SOCS-1 mRNA and SOCS-3 mRNA in the non-MI and MI groups were significantly higher than those in the control group[(0.788±0.101), (0.741±0.111) vs. (0.436±0.044), (P<0.01); (0.841±0.092), (0.776±0.070) vs. (0.454±0.076), (P<0.01)] respectively. The antibody-positive cells of SOCS-1 protein in the myocardium of the non-MI and MI groups were significantly higher than those in the myocardium of the control group[(320.00±48.48), (347.14±70.88) vs. (42.50±10.35), (P<0.01)] respectively. The antibody-positive cells of SOCS-3 protein in the myocardium of the non-MI and MI groups were significantly higher than those in the myocardium of the control group[(381.11±59.25) vs. (40.00±10.69), (P<0.01)] and[(332.86±111.91) vs. (40.00±10.69), (P=0.001)].
The expressions of SOCS-1 and SOCS-3 in the myocardium of patients with SCD from coronary heart disease are significantly increased and contribute to the pathogenesis of SCD.
作为细胞因子的调节剂，细胞因子信号转导抑制因子（SOCS）在炎症反应中发挥着重要作用。一些研究发现 SOCS-1 和 SOCS-3 参与了一些炎症性疾病的发病机制，如类风湿关节炎、炎症性肠病。但 SOCS 在冠心病中的表达尚未报道。本研究旨在探讨猝死者（SCD）心肌中 SOCS-1 和 SOCS-3 的表达及其临床意义。
收集中山大学法医系 2005 年至 2006 年间 24 例猝死者的心肌尸检标本。其中 9 例尸检结果与导致 SCD 的冠状动脉粥样硬化（非心肌梗死）一致（非 MI 组），7 例死于急性心肌梗死（MI 组），8 例死于交通事故和外伤（对照组）。采用 RT-PCR 检测非 MI、MI 和对照组心肌中 SOCS-1mRNA 和 SOCS-3mRNA 的表达。采用免疫组织化学法检测 SOCS-1 和 SOCS-3 蛋白水平。采用 SPSS 版本 13.0 软件进行统计学分析，采用方差分析进行数据分析。
非 MI 和 MI 组的 SOCS-1mRNA 和 SOCS-3mRNA 表达均明显高于对照组[（0.788±0.101），（0.741±0.111）比（0.436±0.044），（P<0.01）；（0.841±0.092），（0.776±0.070）比（0.454±0.076），（P<0.01）]。非 MI 和 MI 组 SOCS-1 蛋白阳性细胞在心肌中的表达明显高于对照组[（320.00±48.48），（347.14±70.88）比（42.50±10.35），（P<0.01）]。非 MI 和 MI 组 SOCS-3 蛋白阳性细胞在心肌中的表达明显高于对照组[（381.11±59.25）比（40.00±10.69），（P<0.01）]和[（332.86±111.91）比（40.00±10.69），（P=0.001）]。
冠心病猝死者心肌中 SOCS-1 和 SOCS-3 的表达明显增加，有助于 SCD 的发病机制。
