Li Xiaoxia, Shang Qing, Zhang Lifan
Department of Rehabilitation, Children's Hospital of Zhengzhou City, Zhengzhou, 450053, Henan, People's Republic of China,
Cell Biochem Biophys. 2014 Dec;70(3):1539-44. doi: 10.1007/s12013-014-0090-6.
To compare the efficacy of cord blood mononuclear cells (MNCs) and CD34+ cells for the treatment of neonatal mice models with cerebral palsy (CP). CP model in neonatal mice was established by the ligation of carotid artery. Mice were randomly designated into MNCs group, CD34+ group, model group and control group (30 mice per group). MNCs and CD34+ cells were isolated from human umbilical cord blood. MNCs were transplanted into mice in the MNCs group and CD34+ cells into mice in the CD34+ group through the jugular vein, respectively. The body weight, histopathology, apoptosis-related gene expression, learning and memory, and motor function of mice in the four groups were compared. Compared with control group, the body weight of mice in model group was significantly lower (P < 0.05). In addition, the right hemisphere was significantly liquefied and voids were found in model mice, in which degeneration and necrosis were found by HE staining. Real-time quantitative fluorescent PCR showed elevated levels of apoptosis-related gene expression and learning and memory function, and motor function were significantly decreased (P < 0.05) in model mice. In the MNCs group and CD34+ group, the weight of mice was significantly increased compared with the model group (P < 0.05). Moreover, neither liquefaction and voids in the hemispheres of mice were found in these two groups, nor degeneration and necrosis of cell. Meanwhile, levels of apoptosis-related gene expression were significantly lower than that of the model group (P < 0.05). Compared with the MNCs group, the expression of apoptotic gene TNF-α and CD40 was significantly lower (P < 0.05). Learning and memory function, and motor function of mice in the MNCs group and CD34+ group were significantly improved than the model group (P < 0.05), and the CD34+ group produced greater improvement than the MNCs group (P < 0.05). MNCs and CD34+ cells can reduce the degree of injury in the neonatal mice with CP. In addition, treatment with MNCs and CD34+ cells suppressed apoptotic gene expression and restored memory and motor function. The efficacy of CD34+ cells after separation and purification was more significant for the treatment of mice with CP.
比较脐血单个核细胞（MNCs）和CD34+细胞治疗新生小鼠脑瘫（CP）模型的疗效。通过结扎颈动脉建立新生小鼠CP模型。将小鼠随机分为MNCs组、CD34+组、模型组和对照组（每组30只小鼠）。从人脐带血中分离出MNCs和CD34+细胞。分别通过颈静脉将MNCs移植到MNCs组小鼠体内，将CD34+细胞移植到CD34+组小鼠体内。比较四组小鼠的体重、组织病理学、凋亡相关基因表达、学习记忆能力和运动功能。与对照组相比，模型组小鼠体重显著降低（P<0.05）。此外，模型小鼠右半球明显液化并有空洞形成，HE染色显示有变性和坏死。实时定量荧光PCR显示，模型小鼠凋亡相关基因表达水平升高，学习记忆功能和运动功能显著下降（P<0.05）。MNCs组和CD34+组小鼠体重与模型组相比显著增加（P<0.05）。此外，这两组小鼠半球均未发现液化和空洞形成，也未发现细胞变性和坏死。同时，凋亡相关基因表达水平显著低于模型组（P<0.05）。与MNCs组相比，凋亡基因TNF-α和CD40的表达显著降低（P<0.05）。MNCs组和CD34+组小鼠的学习记忆功能和运动功能较模型组均有显著改善（P<0.05），且CD34+组的改善程度大于MNCs组（P<0.05）。MNCs和CD34+细胞可减轻新生CP小鼠的损伤程度。此外，MNCs和CD34+细胞治疗可抑制凋亡基因表达，恢复记忆和运动功能。分离纯化后的CD34+细胞治疗CP小鼠的疗效更显著。
