Donmez Ayhan, Yilmaz Fergun, Soyer Nur, Cagirgan Seckin, Arik Bahar, Tombuloglu Murat
Department of Hematology, Ege University Medical School, Izmir, Turkey.
Department of Hematology, Ege University Medical School, Izmir, Turkey.
Transfus Apher Sci. 2014 Oct;51(2):188-92. doi: 10.1016/j.transci.2014.08.013. Epub 2014 Aug 27.
Although peripheral blood stem cell (PBSC) products cryopreserved by non-controlled rate freezing and stored at -80 °C after overnight storage are used frequently, data regarding the rate of loss of CD34+ cells in these products are limited. In this prospective study, CD34+ cells were counted at three (fresh, post-overnight and post-thaw) points in 83 PBSC products from 41 patients by flow cytometry. Compared to fresh products, the mean losses of post-overnight and post-thaw total CD34+ cells are 16.3% and 38.4% (p = 0.02), and the mean losses of post-overnight and post-thaw viable CD34+ cells are 16.5% and 48.5%, respectively (p < 0.001). The numbers of fresh viable, post-thaw total and post-thaw viable CD34+ cells were inversely correlated with the durations of neutrophil and platelet engraftment. Our results indicate that the mean loss of post-thaw total and viable CD34+ cells is approximately 20% higher than that observed in standard cryopreservation methods. In addition, fresh viable, post-thaw total and especially post-thaw viable CD34+ cell levels are valuable predictors of both neutrophil and platelet engraftments.
尽管经常使用通过非控制速率冷冻保存并在过夜储存后于-80°C保存的外周血干细胞（PBSC）产品，但关于这些产品中CD34+细胞损失率的数据有限。在这项前瞻性研究中，通过流式细胞术对41例患者的83份PBSC产品在三个时间点（新鲜、过夜后和解冻后）的CD34+细胞进行计数。与新鲜产品相比，过夜后和解冻后总CD34+细胞的平均损失分别为16.3%和38.4%（p = 0.02），过夜后和解冻后活CD34+细胞的平均损失分别为16.5%和48.5%（p < 0.001）。新鲜活细胞、解冻后总细胞和解冻后活CD34+细胞的数量与中性粒细胞和血小板植入的持续时间呈负相关。我们的结果表明，解冻后总CD34+细胞和活CD34+细胞的平均损失比标准冷冻保存方法中观察到的损失高约20%。此外，新鲜活细胞、解冻后总细胞尤其是解冻后活CD34+细胞水平是中性粒细胞和血小板植入的重要预测指标。
