Antonenas V, Garvin F, Webb M, Sartor M, Bradstock K F, Gottlieb D
Sydney Cellular Therapies Laboratory, Westmead Hospital, Sydney, New South Wales, Australia.
Cytotherapy. 2006;8(2):158-65. doi: 10.1080/14653240600620994.
The optimum conditions for storage and transport of freshly harvested HPC in the liquid state are uncertain. It is not specified in commonly applied standards for stem cell transplantation. We used a viable CD34 assay to determine the optimum temperature for maintaining progenitor cell viability in freshly harvested BM and PBSC. Our aim was to identify standardized conditions for storage and transport of marrow or peripheral blood products that would optimize CD34 recovery, leading to better transplant outcomes.
Samples were aseptically removed from 46 fresh HPC harvests (34 PBSC and 12 BM) and stored at refrigerated temperature (2-8 degrees C), room temperature (18-24 degrees C) and 37 degrees C for up to 72 h. Samples were analyzed for viable CD34+ cells/microL at 0, 24, 48 and 72 h.
The mean viable CD34+ yield prior to storage was 7.7 x 10(6)/kg (range 0.7-30.3). The mean loss of viable CD34+ cells in HPC products at refrigerated temperature was 9.4%, 19.4% and 28% at 24, 48 and 72 h, respectively. In contrast, the mean loss of viable CD34+ cells at room temperature was 21.9%, 30.7% and 43.3% at 24, 48 and 72 h, respectively. No viable CD34+ cells remained after storage at 37 degrees C for 24 h. Only PBSC products and not BM showed temperature-related loss of CD34 viability. Greater loss of viable CD34+ cells was observed for allogeneic PBSC compared with autologous PBSC.
These results demonstrate that the optimum temperature for maintaining the viability of CD34+ cells, during overnight storage and transport of freshly harvested HPC, is 2-8 degrees C. These findings will allow the development of standard guidelines for HPC storage and transport.
新鲜采集的液态造血祖细胞（HPC）的最佳储存和运输条件尚不确定。干细胞移植的常用标准中未对此作出规定。我们使用了一种可行的CD34检测方法来确定在新鲜采集的骨髓和外周血干细胞中维持祖细胞活力的最佳温度。我们的目的是确定骨髓或外周血产品储存和运输的标准化条件，以优化CD34回收率，从而带来更好的移植效果。
从46份新鲜的造血祖细胞采集样本（34份外周血干细胞和12份骨髓）中无菌取出样本，并在冷藏温度（2 - 8摄氏度）、室温（18 - 24摄氏度）和37摄氏度下储存长达72小时。在0、24、48和72小时对样本进行有活力的CD34 +细胞/微升分析。
储存前有活力的CD34 +细胞平均产量为7.7×10（6）/千克（范围为0.7 - 30.3）。在冷藏温度下，造血祖细胞产品中有活力的CD34 +细胞在24、48和72小时的平均损失分别为9.4%、19.4%和28%。相比之下，室温下有活力的CD34 +细胞在24、48和72小时的平均损失分别为21.9%、30.7%和43.3%。在37摄氏度下储存24小时后没有有活力的CD34 +细胞残留。只有外周血干细胞产品而非骨髓显示出与温度相关的CD34活力损失。与自体外周血干细胞相比，异体外周血干细胞中有活力的CD34 +细胞损失更大。
这些结果表明，在新鲜采集的造血祖细胞过夜储存和运输期间，维持CD34 +细胞活力的最佳温度为2 - 8摄氏度。这些发现将有助于制定造血祖细胞储存和运输的标准指南。
