Köller U, Hinterberger W, Gschwandtler L, Grümayer R, Majdic O, Knapp W
Institute of Clinical Chemistry and Laboratory Diagnostics, University of Vienna, Austria.
Blut. 1989 Jan;58(1):21-6. doi: 10.1007/BF00320231.
In patients with severe aplastic anemia (SAA), lymphocyte subpopulations were examined for the presence of HLA-DR and 2H4 (suppressor/inducer subset) antigen-expressing cells by flow cytometric analysis. Investigations were performed on peripheral blood lymphocytes before and after therapy with antithymocyte globulin (ATG) and methylprednisolone (MP), as well as on bone-marrow lymphocytes before therapy. Before treatment, only the absolute numbers of CD4+ T cells and the CD4+HLA-DR+/CD8+HLA-DR+ activated T cell ratio were significantly decreased (p less than 0.01 and p less than 0.001, respectively). Following successful ATG/MP treatment, a decrease in the CD4+/CD8+ T cell ratio was found. Regarding the suppressor/inducer subset, only absolute numbers of CD4+/2H4+ cells were somewhat higher in treated patients; the percentages were the same in all groups of patients. Studies performed on bone-marrow lymphocytes showed significantly decreased percentages of CD4+ and CD8+ T lymphocytes, which also express HLA-DR antigen. No significant changes in the distribution of activated T cells following ATG/MP therapy were found, suggesting that these cells play no major role in the pathogenesis of the disease.
在重型再生障碍性贫血（SAA）患者中，通过流式细胞术分析检测淋巴细胞亚群中表达HLA - DR和2H4（抑制/诱导亚群）抗原的细胞。在使用抗胸腺细胞球蛋白（ATG）和甲基泼尼松龙（MP）治疗前后对外周血淋巴细胞进行研究，同时也对治疗前的骨髓淋巴细胞进行研究。治疗前，仅CD4 + T细胞的绝对数量以及CD4 + HLA - DR + / CD8 + HLA - DR + 活化T细胞比例显著降低（分别为p < 0.01和p < 0.001）。ATG/MP治疗成功后，发现CD4 + / CD8 + T细胞比例降低。关于抑制/诱导亚群，仅治疗患者中CD4 + / 2H4 + 细胞的绝对数量略高；所有患者组中的百分比相同。对骨髓淋巴细胞进行的研究显示，表达HLA - DR抗原的CD4 + 和CD8 + T淋巴细胞百分比显著降低。ATG/MP治疗后活化T细胞的分布未发现显著变化，表明这些细胞在该疾病的发病机制中不起主要作用。
