Lönn U, Lönn S, Nylen U, Winblad G
Radiumhemmet, Karolinska Hospital, Stockholm, Sweden.
Cancer Res. 1989 Mar 1;49(5):1085-9.
5-Fluorouracil and 5-fluorodeoxyuridine induce DNA lesions via two different mechanisms, one involving and the other not involving the incorporation of drug into DNA. The DNA lesions are detected by lysing cells in dilute alkali and then separating the DNA in agarose gel electrophoresis. We examine here the effect of dipyridamole, a nucleoside transport inhibitor, on the DNA lesions. We find that dipyridamole augments the levels of DNA fragmentation when the lesions are induced by the mechanism not involving the incorporation of drug. In parallel cytotoxicity is increased.
5-氟尿嘧啶和5-氟脱氧尿苷通过两种不同机制诱导DNA损伤，一种涉及药物掺入DNA，另一种则不涉及。通过在稀碱中裂解细胞，然后在琼脂糖凝胶电泳中分离DNA来检测DNA损伤。我们在此研究核苷转运抑制剂双嘧达莫对DNA损伤的影响。我们发现，当损伤由不涉及药物掺入的机制诱导时，双嘧达莫会增加DNA片段化水平。同时细胞毒性也会增加。
