Gerfaux J, Sergiescu D, Fournier J G, Pochart F, Joret A M, Chany C
Unité 43 INSERM, Hôpital St. Vincent de Paul, Paris, France.
Cancer Res. 1989 Mar 1;49(5):1241-6.
A stable nonmalignant revertant cell line was derived from Moloney murine sarcoma virus-transformed BALB/c cells after long-term cultivation in the presence of murine type I interferon (IFN). These cells gradually established resistance to exogenous IFN and were also seen to contain IFN-dependent proteins. The presence of an endogenous IFN was confirmed by the results of Northern blot analysis and in situ hybridization with an IFN-beta probe, showing that only mRNA specific for IFN-beta- could be found in the uninduced reverted cells. The latter synthesized only a small amount of IFN-beta protein and exhibited few IFN-specific membrane receptors, which bound recombinant IFN-beta with a high affinity. After treatment with IFN antibody, the overexpression of H-2 major histocompatibility antigen genes was significantly down-regulated. These findings strongly suggest the existence in this reverted cell line of a constitutive IFN which, acting through an autocrine and/or paracrine mechanism, might play a role in maintaining the reverted state.
在存在鼠Ⅰ型干扰素（IFN）的情况下长期培养后，从莫洛尼鼠肉瘤病毒转化的BALB/c细胞中获得了一种稳定的非恶性回复细胞系。这些细胞逐渐对外源IFN产生抗性，并且还发现含有IFN依赖性蛋白。通过Northern印迹分析和用IFN-β探针进行原位杂交的结果证实了内源性IFN的存在，表明在未诱导的回复细胞中仅能发现IFN-β特异性的mRNA。后者仅合成少量的IFN-β蛋白，并且表现出很少的IFN特异性膜受体，这些受体以高亲和力结合重组IFN-β。用IFN抗体处理后，H-2主要组织相容性抗原基因的过表达明显下调。这些发现强烈表明在这种回复细胞系中存在一种组成型IFN，其通过自分泌和/或旁分泌机制发挥作用，可能在维持回复状态中起作用。
