Grassi D, Lagunas N, Calmarza-Font I, Diz-Chaves Y, Garcia-Segura L M, Panzica G C
Cajal Institute, CSIC, Madrid, Spain; University of Torino, Department of Neuroscience "Rita Levi Montalcini", Torino, Italy; Institute of Anatomy and Cell Biology, Department of Molecular Embryology, Albert-Ludwigs-Universität Freiburg, 79104 Freiburg, Germany.
Cajal Institute, CSIC, Madrid, Spain.
Brain Res. 2014 Nov 7;1588:55-62. doi: 10.1016/j.brainres.2014.09.006. Epub 2014 Sep 8.
Arginine-Vasopressin (AVP) may regulate the hypothalamic-pituitary-adrenal axis (HPA) and its effects on depressive responses. In a recent study, we demonstrated that Chronic Unpredictable Stress (CUS) depressive effects are enhanced by long-term ovariectomy (a model of post-menopause). In the present study, we investigated the effects of long-term ovariectomy and CUS on AVP expression in different subdivision of the paraventricular nucleus (PVN) of female mice. Both long-term ovariectomy and CUS affect AVP immunoreactivity in some of the PVN subnuclei of adult female mice. In particular, significant changes on AVP immunoreactivity were observed in magnocellular subdivisions, the paraventricular lateral magnocellular (PaLM) and the paraventricular medial magnocellular (PaMM), the 2 subnuclei projecting to the neurohypophysis for the hormonal regulation of body homeostasis. AVP immunoreactivity was decreased in the PaLM by both the long-term deprivation of ovarian hormones and the CUS. In contrast, AVP immunoreactivity was increased in the PaMM by CUS, whereas it was decreased by ovariectomy. Therefore, present results suggest morphological and functional differences among the PVN's subnuclei and complex interactions among CUS, gonadal hormones and AVP immunoreactivity.
精氨酸加压素（AVP）可能调节下丘脑-垂体-肾上腺轴（HPA）及其对抑郁反应的影响。在最近的一项研究中，我们证明长期卵巢切除术（一种绝经后模型）会增强慢性不可预测应激（CUS）的抑郁作用。在本研究中，我们调查了长期卵巢切除术和CUS对雌性小鼠室旁核（PVN）不同亚区中AVP表达的影响。长期卵巢切除术和CUS均会影响成年雌性小鼠部分PVN亚核中的AVP免疫反应性。特别是，在大细胞亚区，即室旁外侧大细胞（PaLM）和室旁内侧大细胞（PaMM）中观察到AVP免疫反应性有显著变化，这两个亚核投射到神经垂体以进行身体内环境稳态的激素调节。长期缺乏卵巢激素和CUS均会使PaLM中的AVP免疫反应性降低。相比之下，CUS会使PaMM中的AVP免疫反应性增加，而卵巢切除术则会使其降低。因此，目前的结果表明PVN亚核之间存在形态和功能差异，以及CUS、性腺激素和AVP免疫反应性之间存在复杂的相互作用。
