Yoshida Catherine, Lingohr Erika J, Trognitz Friederike, MacLaren Nikki, Rosano Andrea, Murphy Stephanie A, Villegas Andre, Polt Marlies, Franklin Kristyn, Kostic Tanja, Kropinski Andrew M, Card Roderick M
Laboratory for Foodborne Zoonoses, Public Health Agency of Canada (PHAC), Guelph, Ontario, Canada N1G 3W4.
Laboratory for Foodborne Zoonoses, Public Health Agency of Canada (PHAC), Guelph, Ontario, Canada N1G 3W4.
Diagn Microbiol Infect Dis. 2014 Nov;80(3):185-90. doi: 10.1016/j.diagmicrobio.2014.08.006. Epub 2014 Aug 29.
Salmonella serotyping is an essential first step for identification of isolates associated with disease outbreaks. The Salmonella genoserotyping array (SGSA) is a microarray-based alternative to standard serotyping designed to rapidly identify 57 of the most commonly reported serovars through detection of the genes encoding surface O and H antigens and reporting the corresponding serovar in accordance with the existing White-Kaufmann-Le Minor serotyping scheme. In this study, we evaluated the SGSA at 4 laboratories in 3 countries by testing 1874 isolates from human and non-human sources. The SGSA correctly identified 96.7% of isolates from the target 57 serovars. For the prevalent and clinically important Salmonella serovars Enteritidis and Typhimurium, test specificity and sensitivity were greater than 98% and 99%, respectively. Due to its high-throughput nature, the SGSA is a rapid and cost-effective alternative to standard serotyping for identifying the most prevalent serovars of Salmonella.
沙门氏菌血清分型是鉴定与疾病暴发相关分离株的重要第一步。沙门氏菌基因血清分型芯片（SGSA）是一种基于芯片的标准血清分型替代方法，旨在通过检测编码表面O抗原和H抗原的基因，并根据现有的怀特-考夫曼-勒米诺血清分型方案报告相应的血清型，从而快速鉴定57种最常报告的血清型。在本研究中，我们通过检测来自人类和非人类来源的1874株分离株，在3个国家的4个实验室对SGSA进行了评估。SGSA正确鉴定了目标57种血清型中96.7%的分离株。对于流行且具有临床重要性的肠炎沙门氏菌和鼠伤寒沙门氏菌血清型，检测特异性和敏感性分别大于98%和99%。由于其高通量特性，SGSA是一种快速且经济高效的标准血清分型替代方法，用于鉴定沙门氏菌最常见的血清型。
