Pawelec G, Schaudt K, Rehbein A, Busch F W
Immunology Laboratory, Medizinische Klinik, Tübingen, FRG.
Eur J Immunol. 1989 Jan;19(1):197-200. doi: 10.1002/eji.1830190132.
Tumor necrosis factor-alpha (TNF-alpha) is a pleiotropic lymphokine which may have important regulatory effects on immune responses. It is shown here that eight alloreactive CD4+ T cell clones (TCC) secreted significant amounts of TNF-alpha after stimulation with either specific alloantigen or 12-O-tetradecanoylphorbol 13-acetate together with the calcium ionophore ionomycin (up to 50 ng/ml/24 h/10(6) cells) whereas CD8+ TCC failed to do so (max. 2 ng/ml/24 h/10(6) cells). The CD8+ TCC also secreted markedly less granulocyte/macrophage colony-stimulating factor than the CD4+ cells. However, this was not indicative of a general decrease of lymphokine production by CD8+ cells because CD4+ and CD8+ TCC both secreted similar amounts of interferon-gamma. These results show that regulatory CD4+ lymphocytes can produce large amounts of TNF-alpha, whereas CD8+ effector cells cannot do so.
肿瘤坏死因子-α（TNF-α）是一种多效性淋巴因子，可能对免疫反应具有重要的调节作用。本文显示，八个同种异体反应性CD4⁺ T细胞克隆（TCC）在用特异性同种异体抗原或12-O-十四烷酰佛波醇13-乙酸酯与钙离子载体离子霉素刺激后，分泌大量TNF-α（高达50 ng/ml/24 h/10⁶细胞），而CD8⁺ TCC则未能如此（最高2 ng/ml/24 h/10⁶细胞）。CD8⁺ TCC分泌的粒细胞/巨噬细胞集落刺激因子也明显少于CD4⁺细胞。然而，这并不表明CD8⁺细胞的淋巴因子产生普遍减少，因为CD4⁺和CD8⁺ TCC分泌的干扰素-γ量相似。这些结果表明，调节性CD4⁺淋巴细胞可产生大量TNF-α，而CD8⁺效应细胞则不能。
