MacDonald T T, Hutchings P, Choy M Y, Murch S, Cooke A
Department of Paediatric Gastroenterology, St Bartholomew's Hospital, London, England.
Clin Exp Immunol. 1990 Aug;81(2):301-5. doi: 10.1111/j.1365-2249.1990.tb03334.x.
The spot-ELISA technique has been used to enumerate the frequency of cells secreting tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma), isolated from biopsies of normal intestine and from biopsies of children with inflammatory bowel disease. TNF-alpha production was undetectable in six out of 12 biopsies from normal intestine and in the other six biopsies it ranged from 60 to 580 TNF-alpha-secreting cells/10(6) isolated intestinal cells. In contrast, cells isolated from biopsies of children with Crohn's disease (n = 9) all showed elevated frequencies of TNF-alpha-secreting cells (500-12,000 secreting cells/10(6) cells). In ulcerative colitis, four out of eight children had increased production of TNF-alpha and in children with indeterminate colitis two out of three had elevated levels. There was no correlation between plasma TNF-alpha levels and the number of intestinal cells secreting TNF-alpha. In controls and all groups of patients IFN-gamma-secreting cells were uncommon. These results suggest that TNF-alpha is an important mediator of inflammation in the human gut, and, furthermore, may play a role in the growth failure frequently seen in children with inflammatory bowel disease.
斑点酶联免疫吸附测定技术已用于对从正常肠活检组织以及炎性肠病患儿的活检组织中分离出的分泌肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）的细胞频率进行计数。在12份正常肠活检组织中有6份未检测到TNF-α的产生，在另外6份活检组织中，TNF-α分泌细胞的频率为60至580个/10⁶个分离出的肠细胞。相比之下，从克罗恩病患儿（n = 9）的活检组织中分离出的细胞均显示TNF-α分泌细胞的频率升高（500 - 12,000个分泌细胞/10⁶个细胞）。在溃疡性结肠炎中，8名儿童中有4名TNF-α产生增加，在不确定性结肠炎患儿中，3名中有2名水平升高。血浆TNF-α水平与分泌TNF-α的肠细胞数量之间无相关性。在对照组和所有患者组中，分泌IFN-γ的细胞并不常见。这些结果表明，TNF-α是人类肠道炎症的重要介质，此外，可能在炎性肠病患儿中常见的生长发育迟缓中起作用。
