Giuffrida M G, Villalta D, Mistrello G, Amato S, Asero R
Istituto Scienze Produzioni Alimentari CNR, c/o BioIndustry Park S. Fumero, Colleretto Giacosa, (TO), Italy.
Allergologia e Immunologia Clinica, Dipartimento di Medicina di Laboratorio, A.O. "S. Maria degli Angeli", Pordenone, Italy.
Eur Ann Allergy Clin Immunol. 2014 Sep;46(5):172-7.
Little is known about the prevalence and clinical relevance of sensitization to shrimp allergens other than tropomyosin.
We detected the prevalence of arginine kinase and sarcoplasmic calcium binding protein sensitization, and identified a high molecular weight allergen that is frequently recognized by Italian shrimp-allergic patients.
Sera from 40 shrimp-allergic patients underwent the detection of IgE specific for arginine kinase (rPen m 2) and sarcoplasmic calcium-binding protein (rPen m 4) by ISAC 112 Microarray platform and immunoblot analysis. A high molecular weight shrimp allergen was identified by N-terminal amino acid sequencing.
IgE to rPen m 2 and rPen m 4 were found in 4/40 (10%) and 6/40 (15%) sera, respectively; two sera reacted to both allergens. Clinically, 6/8 Pen m 2 and/or Pen m 4 reactors experienced severe allergies to shrimp. On immunoblot, 4/6 rPen m 4-positive sera showed IgE reactivity at about 20 kDa, whereas no rPen m 2-positive serum reacted at about 40 kDa. Nineteen (47%) sera showed IgE reactivity at molecular weights > 60 kDa. Such profile was not associated with IgE reactivity to rPen m 2 or rPen m 4. N-terminal amino acid sequencing of the high molecular weight allergen led to the identification of hemocyanin.
Shrimp arginine kinase and sarcoplasmic calcium-binding protein are minor allergens sensitizing only 10%-15% of Italian shrimp-allergic patients, but are clinically relevant. Hemocyanin is a clinically relevant high molecular weight shrimp allergen possibly cross-reacting to house dust mite.
除原肌球蛋白外，对虾类过敏原致敏的患病率及临床相关性知之甚少。
我们检测了精氨酸激酶和肌浆钙结合蛋白致敏的患病率，并鉴定出一种意大利虾类过敏患者中经常识别的高分子量过敏原。
通过ISAC 112微阵列平台和免疫印迹分析，对40例虾类过敏患者的血清进行精氨酸激酶（rPen m 2）和肌浆钙结合蛋白（rPen m 4）特异性IgE检测。通过N端氨基酸测序鉴定一种高分子量虾类过敏原。
分别在4/40（10%）和6/40（15%）的血清中发现了针对rPen m 2和rPen m 4的IgE；两份血清对两种过敏原均有反应。临床上，6/8例对Pen m 2和/或Pen m 4有反应的患者对虾有严重过敏反应。在免疫印迹中，4/6例rPen m 4阳性血清在约20 kDa处显示IgE反应性，而没有rPen m 2阳性血清在约40 kDa处有反应。19份（47%）血清在分子量>60 kDa处显示IgE反应性。这种情况与对rPen m 2或rPen m 4的IgE反应性无关。高分子量过敏原的N端氨基酸测序鉴定出了血蓝蛋白。
虾类精氨酸激酶和肌浆钙结合蛋白是次要过敏原，仅使10%-15%的意大利虾类过敏患者致敏，但具有临床相关性。血蓝蛋白是一种具有临床相关性的高分子量虾类过敏原，可能与屋尘螨发生交叉反应。
