Morrissey P J, Goodwin R G, Nordan R P, Anderson D, Grabstein K H, Cosman D, Sims J, Lupton S, Acres B, Reed S G
Immunex Corporation, Seattle, Washington 98101.
J Exp Med. 1989 Mar 1;169(3):707-16. doi: 10.1084/jem.169.3.707.
The activation of highly purified murine peripheral T cells in vitro by Con A is dependent on a co-stimulatory signal that is not IL-1 or IL-2. Previous evidence has demonstrated that the recently defined lymphokine IL-6 could provide costimulatory activity for purified T cells cultured with Con A. In this report we demonstrate that IL-7 also has potent co-stimulatory activity for purified murine T cells, as well as its previously described ability to support the growth of pre-B cells in Witte-Whitlock cultures. When rIL-7 was added to cultures of purified T cells together with Con A, it induced the expression of IL-2 receptors, IL-2 production, and consequently proliferation. In addition, IL-7 exhibited the same magnitude of activity in this assay as IL-6. Also, anti-IL-6 antibody which inhibited the IL-6-induced response had no effect on the IL-7 response. Thus, IL-7 does not act by inducing IL-6. These results demonstrate that IL-7, a potent growth stimulus for pre-B cells, also has a role in T cell activation.
伴刀豆球蛋白A（Con A）在体外对高度纯化的小鼠外周T细胞的激活依赖于一种不是白细胞介素-1（IL-1）或白细胞介素-2（IL-2）的共刺激信号。先前的证据表明，最近定义的淋巴因子IL-6可以为用Con A培养的纯化T细胞提供共刺激活性。在本报告中，我们证明IL-7对纯化的小鼠T细胞也具有强大的共刺激活性，以及其先前描述的在维特-惠特洛克培养物中支持前B细胞生长的能力。当将重组白细胞介素-7（rIL-7）与Con A一起添加到纯化T细胞的培养物中时，它诱导了IL-2受体的表达、IL-2的产生，并因此导致了增殖。此外，在该试验中，IL-7表现出与IL-6相同程度的活性。而且，抑制IL-6诱导反应的抗IL-6抗体对IL-7反应没有影响。因此，IL-7不是通过诱导IL-6起作用的。这些结果表明，IL-7作为前B细胞的一种强大生长刺激因子，在T细胞激活中也发挥作用。
