从人T细胞白血病中纯化白细胞介素2至均一性并进行部分特性鉴定。
Purification to homogeneity and partial characterization of interleukin 2 from a human T-cell leukemia.
作者信息
Stern A S, Pan Y C, Urdal D L, Mochizuki D Y, DeChiara S, Blacher R, Wideman J, Gillis S
出版信息
Proc Natl Acad Sci U S A. 1984 Feb;81(3):871-5. doi: 10.1073/pnas.81.3.871.
Abstract
A method utilizing reversed-phase high-performance liquid chromatography has been developed for the purification to homogeneity of interleukin 2 (IL-2) isolated from a human T-cell leukemia. A final purification of 500,000-fold was obtained with a specific activity of pure IL-2 of 10(9) units/mg. The amino acid analysis of natural IL-2 is strikingly similar to the composition deduced from sequence analysis of a cDNA coding for human IL-2. Protein sequence analysis of CNBr-derived peptides yields data consistent with the sequence proposed from cloned cDNA. The availability of homogeneous IL-2 will allow accurate biological studies of its activity free from the contamination of the numerous lymphokine species that are known to be co-produced with IL-2 during the induction procedure.
摘要
已开发出一种利用反相高效液相色谱法从人T细胞白血病中分离白细胞介素2（IL-2）并将其纯化至同质的方法。最终获得了50万倍的纯化效果，纯化后的IL-2比活性为10⁹单位/毫克。天然IL-2的氨基酸分析结果与从编码人IL-2的cDNA序列分析推导的组成惊人地相似。对溴化氰衍生肽段进行蛋白质序列分析得到的数据与克隆cDNA所提出的序列一致。纯质IL-2的可得性将有助于对其活性进行准确的生物学研究，而不会受到诱导过程中已知与IL-2共同产生的众多淋巴因子种类的污染。
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本文引用的文献
1
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J Biol Chem. 1962 Jun;237:1856-60.
2
A gas-liquid solid phase peptide and protein sequenator.一种气-液-固相肽和蛋白质测序仪。
J Biol Chem. 1981 Aug 10;256(15):7990-7.
3
Microsequence analysis of peptides and proteins. II. Separation of amino acid phenylthiohydantoin derivatives by high-performance liquid chromatography on octadecylsilane supports.肽与蛋白质的微量序列分析。II. 用十八烷基硅烷载体上的高效液相色谱法分离氨基酸苯硫代乙内酰脲衍生物
Anal Biochem. 1982 Mar 1;120(2):302-11. doi: 10.1016/0003-2697(82)90351-7.
4
Biochemical separation of a human B cell mitogenic factor.人B细胞促有丝分裂因子的生化分离
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5
6
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7
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8
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