Anahita Shaer,Islamic Azad University, Fars Science & Research Branch, Fars, Iran. Transplant research Center, Shiraz University of Medical Science, Shiraz, Iran.
Negar Azarpira, Transplant research Center, Shiraz University of Medical Science, Shiraz, Iran.
Pak J Med Sci. 2014 Sep;30(5):1022-6. doi: 10.12669/pjms.305.4537.
Mesenchymal stromal cells (MSCs) are considered as an excellent source in regenerative medicine, but availability and ethical problems limited their routine use. Therefore, another available source with easy procedure and exempt from ethical debate is important. The purpose of this study is to isolate and characterize the MSCs from human placenta. The stromal cells were isolated from Placental Decidua Basalis (PDB-MSC), Umbilical cord Wharton's Jelly (WJ-MSC) and Amniotic Membrane (AM-MSC).
Full term human placentas (n=4), from cesarean section delivery were collected. Small fragments from different parts were cultures as explants. The immunophenotyping, mesodermal differentiation, growth kinetics and stemness gene expression was studied.
The cultivated cells from three sources expressed CD44, CD105, and CD90. Gene expression of NANOG and OCT4 confirmed the undifferentiated state. The doubling-times for WJ-MSCs, PLC-MSCs and AM-MSCs, respectively, were 21±8h, 28±9h and 25±9h at passage three and 30±5h, 45±7h and 45±7h at passage tenth. The proliferative potential of WJ-MSCs tended to be higher than the other two sources.
The fetal derives stromal cells; especially the early passages of WJ-MSCs are available supplies for large scale production of MSC for using in clinical studies or research projects.
间充质干细胞（MSCs）被认为是再生医学的极佳来源，但可用性和伦理问题限制了其常规应用。因此，寻找另一种来源丰富、程序简单且不存在伦理争议的细胞来源非常重要。本研究旨在从人胎盘分离和鉴定间充质干细胞。基质细胞是从胎盘蜕膜基质（PDB-MSC）、脐带华通氏胶（WJ-MSC）和羊膜（AM-MSC）中分离出来的。
收集 4 例剖宫产足月胎盘，从小的胎盘碎片培养成组织块。对细胞进行免疫表型、中胚层分化、生长动力学和干性基因表达研究。
三种来源的培养细胞均表达 CD44、CD105 和 CD90。NANOG 和 OCT4 的基因表达证实了未分化状态。WJ-MSCs、PLC-MSCs 和 AM-MSCs 的倍增时间分别为第 3 代时的 21±8h、28±9h 和 25±9h，第 10 代时的 30±5h、45±7h 和 45±7h。WJ-MSCs 的增殖潜力似乎高于其他两种来源。
胎儿来源的基质细胞，尤其是 WJ-MSCs 的早期传代细胞，是用于临床研究或研究项目中大规模 MSC 生产的可用供应品。
