显色琼脂培养基与传统培养系统对尿病原菌分离和初步鉴定的比较评价。
Comparative evaluation of chromogenic agar medium and conventional culture system for isolation and presumptive identification of uropathogens.
机构信息
Laila Akter, M.Phil (Microbiol), Associate Professor, Department of Microbiology, Islami Bank Medical College, Rajshahi, Bangladesh.
Rezwana Haque, M.Phil (Microbiol), Assistant Professor, Islami Bank Medical College, Rajshahi, Bangladesh.
出版信息
Pak J Med Sci. 2014 Sep;30(5):1033-8. doi: 10.12669/pjms.305.5243.
OBJECTIVE
Urine is the most frequent specimen received for culture/sensitivity by clinical laboratories. The microbiological performance of HiCrome UTI agar medium was compared with Blood agar and MacConkey agar for isolation and presumptive identification of bacteria from urine culture.
METHODS
A total of 443 consecutively collected midstream and/or catheter-catch urine samples from patients attending the Islami Bank Medical College Hospital, Rajshahi, Bangladesh during January to December, 2012 were cultured. Urine samples showing pus cells ≥ 5/HPF were inoculated on to Blood agar (BA), MacConkey agar (MAC) and HiCrome UTI agar (CA) media simultaneously and incubated overnight aerobically at 37(0)C. Rate of isolation and presumptive identification of bacterial species were compared for different media.
RESULTS
Culture yielded a total of 199 bacterial isolates from 189 (42.67%) positive plates including 179 (40.40%) unimicrobial and 10 (2.26%) polymicrobial (mixed growth of pair of bacteria) growths. Both HiCrome UTI agar and Blood agar media supported 100% growths while 151 (75.88%) growths were observed on MacConkey agar. The rate of presumptive identification was found significantly higher on HiCrome UTI agar (97.49%) than MAC agar (67.34%) (P<0.001) as primary urine culture medium. Of 199 isolates, E. coli was found to be the leading uropathogen isolated from 118 (59.30%) samples with its presumptive identification rate of 95.76%, 93.22% and 5.93% on CA, MAC and BA respectively. All 10 (100%) polymicrobial growths were demonstrated distinctly on CA against only 01(10%) on each BA and MAC.
CONCLUSION
HiCrome UTI agar was found to be more useful as primary urine culture medium in both higher rate of isolation and presumptive identification of uropathogens in comparison to conventional media. Its inherent characteristics in demonstrating polymicrobial growth and ease of rapid identification by distinct colony colour are unique.
目的
尿液是临床实验室最常收到进行培养/药敏的标本。本研究比较了 HiCrome UTI 琼脂培养基与血琼脂和麦康凯琼脂在分离和推定鉴定尿液培养物中的细菌方面的微生物性能。
方法
2012 年 1 月至 12 月，从孟加拉国拉杰沙希伊斯兰银行医学院医院就诊的连续 443 例中段尿和/或导管采集尿样进行培养。将白细胞≥ 5/HPF 的尿液样本同时接种到血琼脂（BA）、麦康凯琼脂（MAC）和 HiCrome UTI 琼脂（CA）培养基上，在 37°C 下有氧孵育过夜。比较不同培养基的细菌分离和推定鉴定率。
结果
培养共从 189 个阳性平板（42.67%）中获得 199 株细菌分离株，包括 179 株（40.40%）单一微生物和 10 株（2.26%）多微生物（两种细菌的混合生长）。HiCrome UTI 琼脂和血琼脂均支持 100%的生长，而麦康凯琼脂则观察到 151 株（75.88%）生长。作为原发性尿液培养，HiCrome UTI 琼脂（97.49%）的推定鉴定率明显高于 MAC 琼脂（67.34%）（P<0.001）。199 株分离株中，大肠杆菌是从 118 份（59.30%）样本中分离出的主要尿路病原体，其推定鉴定率分别为 CA 95.76%、MAC 93.22%和 BA 5.93%。所有 10 株（100%）多微生物生长均在 CA 上明显显示，而在 BA 和 MAC 上仅分别显示 01 株（10%）。
结论
与传统培养基相比，HiCrome UTI 琼脂作为原发性尿液培养物，在提高尿路病原体的分离率和推定鉴定率方面更有用。其在显示多微生物生长和通过独特的菌落颜色进行快速鉴定方面的固有特性是独特的。
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