使用经多羟基-C60、葡萄糖氧化酶和离子液体修饰的玻碳电极进行葡萄糖生物传感。
Glucose biosensing using glassy carbon electrode modified with polyhydroxy-C60, glucose oxidase and ionic-liquid.
作者信息
Yang Tian, Yang Xiao-Lu, Zhang Yu-Shuai, Xiao BaoLin, Hong Jun
机构信息
School of Life Sciences, Henan University, Kaifeng 475000, China.
School of Life Sciences, Henan University, Kaifeng 475000, China Institute of Biotechnology, Henan University, Kaifeng 475000, China.
出版信息
Biomed Mater Eng. 2014;24(6):2197-202. doi: 10.3233/BME-141031.
Direct electrochemistry of glucose oxidase (GOD) was achieved when an ionic liquid/GOD-Polyhydroxy-C60 functional membrane was confined on a glassy carbon electrode (GCE). The cyclic voltammograms (CVs) of the modified GCE showed a pair of redox peaks with a formal potential (E°') of - 329 ± 2 mV. The heterogeneous electron transfer constant (k(s)) was 1.43 s-1. The modified GCE response to glucose was linear in the range from 0.02 to 2.0 mM. The detection limit was 1 μM. The apparent Michaelis-Menten constant (K(m)(app)) was 1.45 mM.
当离子液体/葡萄糖氧化酶-多羟基-C60功能膜被限制在玻碳电极（GCE）上时，实现了葡萄糖氧化酶（GOD）的直接电化学。修饰后的玻碳电极的循环伏安图（CVs）显示出一对形式电位（E°'）为-329±2 mV的氧化还原峰。异相电子转移常数（k(s)）为1.43 s-1。修饰后的玻碳电极对葡萄糖的响应在0.02至2.0 mM范围内呈线性。检测限为1 μM。表观米氏常数（K(m)(app)）为1.45 mM。
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