School of Chemical and Material Engineering, Jiangnan University, Wuxi, China.
Biosens Bioelectron. 2010 Feb 15;25(6):1434-8. doi: 10.1016/j.bios.2009.10.045. Epub 2009 Nov 6.
The paper describes an ingenious approach for the fabrication of a promising glucose sensor, GOx/C(60)-Fc-CS-IL, that exploits the synergistic beneficial characteristics of fullerene (C(60)), ferrocene (Fc), chitosan (CS) and ionic liquid (IL) for glucose oxidase (GOx). Cyclic voltammetry, impedance spectroscopy and chronoamperometry were used to evaluate performance of the biosensor, respectively. Since efficient electron transfer between GOx and the electrode was achieved, the biosensor exhibits a high sensitivity (234.67 nA nM(-1) cm(-2)), low detection limit (S/N=3) (3x10(-9) M), fast response time (0.752 s), wide calibration range (from 1x10(-8) M to 1x10(-5) M) and excellent long-term stability (30 weeks). The apparent Michaelis-Menten constant (K(M)) of GOx on the composite medium, 0.03 mM, shows high bioelectrocatalytic activity of immobilized enzyme toward glucose oxidation. Due to low operating potential (100 V), the biosensor is relatively insensitive to electroactive interfering species in human blood such as ascorbic acid, and uric acid, which are commonly found in blood samples. Excellent electrochemical reversibility, high sensitivity and stability, technically simple and possibility of preparation at short period of time are of great advantages of these glucose biosensors.
该论文描述了一种巧妙的方法，用于制造一种有前途的葡萄糖传感器 GOx/C(60)-Fc-CS-IL，该传感器利用富勒烯 (C(60))、二茂铁 (Fc)、壳聚糖 (CS) 和离子液体 (IL) 的协同有益特性来固定葡萄糖氧化酶 (GOx)。循环伏安法、阻抗谱和计时安培法分别用于评估生物传感器的性能。由于 GOx 和电极之间实现了有效的电子转移，因此该生物传感器表现出高灵敏度 (234.67 nA nM(-1) cm(-2))、低检测限 (S/N=3) (3x10(-9) M)、快速响应时间 (0.752 s)、宽校准范围 (从 1x10(-8) M 到 1x10(-5) M) 和出色的长期稳定性 (30 周)。固定化酶对葡萄糖氧化的高生物电化学催化活性表现为复合介质上 GOx 的表观米氏常数 (K(M)) 为 0.03 mM。由于工作电位低 (100 V)，该生物传感器对血液中常见的电活性干扰物质如抗坏血酸和尿酸相对不敏感。这些葡萄糖生物传感器具有电化学可逆性好、灵敏度和稳定性高、技术简单以及可在短时间内制备等优点。
