Tong Y, Netchitaïlo P, Leboulenger F, Vaudry H, Pelletier G
MRC Group in Molecular Endocrinology, Le Centre Hospitalier de l'Université Laval, Québec, Canada.
J Comp Neurol. 1989 Mar 15;281(3):384-96. doi: 10.1002/cne.902810305.
The distribution of atrial natriuretic factor (ANF) binding sites was investigated in the central nervous system of the frog Rana ridibunda using the technique of in vitro receptor autoradiography by means of [125I]-labeled ANF-28. The anatomic distribution of ANF recognition sites was determined on Kodak ARX films apposed onto tissue sections, and their distribution was examined in greater detail by analysis of autoradiograms generated by using emulsion-coated sections. The highest levels of ANF binding sites were found in the olfactory bulb, the dorsal pallium, the septum, the habenular nucleus, the dorsal infundibular nucleus, the interpeduncular nucleus, and in the tectum. Moderate levels of ANF binding sites were observed in the thalamus and throughout the mesencephalon, whereas low levels were detected in the lateral and medial pallium, the medial forebrain bundle, and the nucleus rotondus. In the pituitary gland, the neural and distal lobes were densely loaded with ANF binding sites, whereas no autoradiographic labeling was observed in the pars intermedia. In general, there was a good correlation between the location of ANF receptors and the distribution of ANF-containing neurons, as previously determined by immunocytochemistry. Together these results support the view that ANF may act as a neurotransmitter or a neuromodulator in various regions of the frog brain.
利用[125I]标记的心房钠尿肽-28，采用体外受体放射自显影技术，研究了牛蛙中枢神经系统中心房钠尿肽（ANF）结合位点的分布。在贴于组织切片上的柯达ARX胶片上确定ANF识别位点的解剖分布，并通过分析用乳胶包被切片产生的放射自显影片更详细地检查其分布。在嗅球、背侧大脑皮质、隔膜、缰核、背侧漏斗核、脚间核和中脑发现了最高水平的ANF结合位点。在丘脑和整个中脑观察到中等水平的ANF结合位点，而在外侧和内侧大脑皮质、内侧前脑束和圆核中检测到低水平的ANF结合位点。在垂体中，神经叶和远侧叶密集地分布着ANF结合位点，而在中间部未观察到放射自显影标记。总体而言，如先前通过免疫细胞化学所确定的，ANF受体的位置与含ANF神经元的分布之间存在良好的相关性。这些结果共同支持了这样一种观点，即ANF可能在蛙脑的各个区域作为神经递质或神经调质发挥作用。
