Vallarino M, Goula D, Trabucchi M, Masini M A, Chartrel N, Vaudry H
Institute of Comparative Anatomy, University of Genova, Italy.
J Comp Neurol. 1996 Nov 18;375(3):345-62. doi: 10.1002/(SICI)1096-9861(19961118)375:3<345::AID-CNE1>3.0.CO;2-2.
The localization of atrial natriuretic factor (ANF)-immunoreactive elements was investigated in the brain of the African lungfish, Protopterus annectens, by using antisera raised against rat and human ANF(1-28). Concurrently, the distribution of ANF binding sites was studied by autoradiography using radioiodinated human ANF(1-28) as a tracer. In general, there was a good correlation between the distribution of ANF-immunoreactive structures and the location of ANF binding sites in several areas of the brain, particularly in the ventral part of the medial subpallium, the rostral preoptic region, the preoptic periventricular nucleus, the caudal hypothalamus, the neural lobe of the pituitary, and the mesencephalic tectum. In contrast, mismatching was observed in the pallium (which contained a high density of binding sites and a low concentration of ANF immunoreactive elements) as well as in the lateral subpallium and the medial region of the ventral thalamus, in which a low concentration of binding sites but a high density of ANF-immunoreactive fibers were detected. The present data provide the first localization of ANF-related peptides in the brain of dipnoans and the first anatomical distribution of ANF binding sites in the brain of fish. The results show that the ANF peptidergic systems of P. annectens exhibit similarities with those previously described in the frog Rana ridibunda, supporting the existence of relationships between dipnoans and amphibians. The location of ANF-like immunoreactivity and the distribution of ANF binding sites suggest that ANF-related peptides may act as hypothalamic neurohormones as well as neurotransmitters and/or neuromodulators in the lungfish brain.
利用针对大鼠和人ANF(1 - 28)产生的抗血清，研究了非洲肺鱼（Protopterus annectens）脑中心房钠尿肽（ANF）免疫反应性成分的定位。同时，使用放射性碘化人ANF(1 - 28)作为示踪剂，通过放射自显影研究了ANF结合位点的分布。总体而言，在脑的几个区域，特别是内侧皮质下区腹侧、视前区前部、视前室周核、下丘脑尾部、垂体神经叶和中脑顶盖，ANF免疫反应性结构的分布与ANF结合位点的位置之间存在良好的相关性。相比之下，在大脑皮层（含有高密度的结合位点和低浓度的ANF免疫反应性成分）以及外侧皮质下区和腹侧丘脑内侧区域观察到不匹配，在这些区域检测到低浓度的结合位点但高密度的ANF免疫反应性纤维。本研究数据首次在肺鱼脑中定位了ANF相关肽，并首次给出了鱼类脑中ANF结合位点的解剖学分布。结果表明，非洲肺鱼的ANF肽能系统与先前在泽蛙（Rana ridibunda）中描述的系统具有相似性，支持了肺鱼和两栖动物之间存在关联。ANF样免疫反应性的位置和ANF结合位点的分布表明，ANF相关肽可能在肺鱼脑中作为下丘脑神经激素以及神经递质和/或神经调节剂发挥作用。
