Ujita Minoru, Koike Shota, Yamauchi Yosuke, Kiochi Noburo, Yura Hiroki, Yamanaka Mayumi, Okumura Hiroki
a Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agriculture , Meijo University, Tempaku-ku , Nagoya 468-8502 , Japan.
Biosci Biotechnol Biochem. 2014;78(7):1203-5. doi: 10.1080/09168451.2014.915734. Epub 2014 May 29.
Human macrophage dectin-1, a type II transmembrane β-glucan receptor, was expressed as a fusion protein with an N-terminal hexahistidine tag in a baculovirus-silkworm expression system and assayed for binding activity. Recombinant dectin-1 specifically bound to some β-glucans, and the neck domain and N-linked oligosaccharide chains of human dectin-1 did not affect the ligand binding activity and specificity of the receptor.
人巨噬细胞dectin-1是一种II型跨膜β-葡聚糖受体，在杆状病毒-家蚕表达系统中作为带有N端六组氨酸标签的融合蛋白表达，并检测其结合活性。重组dectin-1特异性结合某些β-葡聚糖，人dectin-1的颈部结构域和N-连接寡糖链不影响该受体的配体结合活性和特异性。
