Mayumi M, Kawabe T, Nishioka H, Tanaka M, Kim K M, Heike T, Yodoi J, Mikawa H
Department of Pediatrics, Faculty of Medicine, Kyoto University, Japan.
Mol Immunol. 1989 Mar;26(3):241-7. doi: 10.1016/0161-5890(89)90077-1.
The regulation of low affinity IgE receptor (Fc epsilon R2/CD23) expression on the human monoblast cell line U937 was examined by an anti-Fc epsilon R2/CD23 monoclonal antibody (H107) and the cDNA probe for Fc epsilon R2/CD23. Alpha interferon (IFN-alpha) and its intracellular mediator, (2'-5')oligoadenylate (2, 5-A), induced Fc epsilon R2/CD23 expression on U937 with no significant increase of the Fc epsilon R2/CD23 mRNA. PMA and IFN-gamma increased both surface Fc epsilon R2/CD23 expression and the Fc epsilon R2/CD23 mRNA levels. IFN-alpha effectively induced 2, 5-A synthetase activity in U937 cells, whereas IFN-gamma induced little. The results suggest that the mechanisms of enhancement of Fc epsilon R2/CD23 expression on U937 cells by IFN-alpha and IFN-gamma are different and that 2, 5-A may play an important role in the Fc epsilon R2/CD23 expression on U937 cells induced by IFN-alpha.
利用抗FcεR2/CD23单克隆抗体（H107）和FcεR2/CD23的cDNA探针，研究了人单核细胞系U937上低亲和力IgE受体（FcεR2/CD23）表达的调控。α干扰素（IFN-α）及其细胞内介质（2'-5'）寡腺苷酸（2,5-A）诱导U937上FcεR2/CD23的表达，而FcεR2/CD23 mRNA无显著增加。佛波酯（PMA）和IFN-γ增加了表面FcεR2/CD23的表达以及FcεR2/CD23 mRNA水平。IFN-α有效诱导U937细胞中的2,5-A合成酶活性，而IFN-γ诱导的很少。结果表明，IFN-α和IFN-γ增强U937细胞上FcεR2/CD23表达的机制不同，并且2,5-A可能在IFN-α诱导的U937细胞FcεR2/CD23表达中起重要作用。
