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从牛、羊和驴粪便中分离网尾线虫属的贝尔曼氏分离法。

Baermannization of Dictyocaulus spp. from faeces of cattle, sheep and donkeys.

作者信息

Rode B, Jørgensen R J

机构信息

Department of Internal Medicine, Royal Veterinary and Agricultural University, Copenhagen, Denmark.

出版信息

Vet Parasitol. 1989 Jan;30(3):205-11. doi: 10.1016/0304-4017(89)90016-2.

Abstract

Faeces were collected per rectum from calves infected with Dictyocaulus viviparus (D.v.), from lambs infected with Dictyocaulus filaria (D.f.) and donkeys infected with Dictyocaulus arnfieldi (D.a.). In one experiment, the influence of storage temperature before Baermannization was investigated. Recovery rate for D.v. was approximately 80% after 24 h at 4 degrees C or 16 degrees C but only 40% at 20 degrees C. After two days at 20 degrees C the rate had fallen to 20%. Recovery rates for D.f. decreased so markedly during the first 12 h at 4, 16 and 20 degrees C that storage can not be recommended. Losses in the recovery rates of D.a. appeared insignificant after 48 h at 4 degrees C but not at 16 degrees C and 20 degrees C. In experiment II the time taken for larvae to emerge from a 10-g sample as well as the sedimentation time in Baermann tubes was investigated. The bulk of the D.v. larvae remained in the faeces for about 10 h whereas D.f. larvae emerged during the first few hours. D.a. larvae were intermediate in this respect. Sedimentation of the bulk of larvae from all three species took place within a few hours with the modified Baermann technique used.

摘要

从感染胎生网尾线虫(D.v.)的犊牛、感染丝状网尾线虫(D.f.)的羔羊以及感染安氏网尾线虫(D.a.)的驴的直肠采集粪便。在一项实验中,研究了贝曼氏分离法之前粪便储存温度的影响。D.v.在4℃或16℃下储存24小时后的回收率约为80%,但在20℃下仅为40%。在20℃下储存两天后,回收率降至20%。D.f.在4℃、16℃和20℃下最初12小时内回收率显著下降,因此不建议储存。D.a.在4℃下储存48小时后回收率损失似乎不显著,但在16℃和20℃下则不然。在实验II中,研究了幼虫从10克样本中孵出所需的时间以及在贝曼氏管中的沉淀时间。大多数D.v.幼虫在粪便中停留约10小时,而D.f.幼虫在最初几小时内孵出。D.a.幼虫在这方面处于中间状态。使用改良贝曼氏技术,所有三种线虫的大多数幼虫在几小时内完成沉淀。

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