Jørgensen R J, Andersen S
Nord Vet Med. 1984 May-Jun;36(5-6):162-9.
Between 10 and 25% of the Dictyocaulus arnfieldi larvae excreted in faeces from a naturally infected donkey were harvested as infective stages from faecal cultures by means of Pilobolus fungi. The faeces were collected between 24 and 56 hours after drenching the donor animal with Pilobolus spores and kept at 16 +/- 2 degrees C. Most larvae were collected between the 5th and the 8th day of culturing during which period fructification and sporangium discharge also peaked. The sporangia and the adhering larvae were collected in Petri dishes inserted between the faecal mass and a light source. All recovered larvae were viable. A mean larval length of 368 microns (range 312-440 microns) and width of 14.6 microns (range 12-20 microns) was recorded for the infective stage. The method was found suitable for the recovery of infective stages for experimental purposes. The authors suggest that the Pilobolus mechanism play an important part in the spread of equine lungworm infection under field conditions similar to the situation in bovine lungworm (Dictyocaulus viviparus) infection.
从自然感染的驴粪便中排出的10%至25%的阿诺菲迪毛线虫幼虫，通过毛霉真菌从粪便培养物中收获作为感染阶段。在给供体动物投喂毛霉孢子后24至56小时收集粪便，并保持在16±2摄氏度。大多数幼虫在培养的第5天至第8天收集，在此期间结实和孢子囊排放也达到峰值。孢子囊和附着的幼虫收集在插入粪便团和光源之间的培养皿中。所有回收的幼虫均存活。记录到感染阶段幼虫的平均长度为368微米（范围312 - 440微米），宽度为14.6微米（范围12 - 20微米）。该方法被认为适用于为实验目的回收感染阶段。作者认为，在类似于牛肺线虫（胎生网尾线虫）感染的野外条件下，毛霉机制在马肺线虫感染的传播中起重要作用。
