采用连接酶介导的实时 PCR 高分辨率熔解曲线分析方法快速进行 ESKAPE（屎肠球菌、金黄色葡萄球菌、肺炎克雷伯菌、鲍曼不动杆菌、铜绿假单胞菌和肠杆菌属）病原体的流行病学分型评估。
Evaluation of high-resolution melting curve analysis of ligation-mediated real-time PCR, a rapid method for epidemiological typing of ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter Species) pathogens.
机构信息
Department of Clinical Microbiology, Kalmar County Hospital, Kalmar, Sweden Department of Medicine and Optometry, Linnaeus University, Kalmar, Sweden
Department of Clinical Microbiology and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
出版信息
J Clin Microbiol. 2014 Dec;52(12):4339-42. doi: 10.1128/JCM.02537-14. Epub 2014 Sep 17.
Abstract
A single-tube method, ligation-mediated real-time PCR high-resolution melt analysis (LMqPCR HRMA), was modified for the rapid typing of Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp. (ESKAPE) pathogens. A 97% agreement (60/62 isolates) was achieved in comparison to pulsed-field gel electrophoresis (PFGE) results, which indicates that LMqPCR HRMA is a rapid and accurate screening tool for monitoring nosocomial outbreaks.
摘要
一种单管方法，连接介导的实时 PCR 高分辨率熔解分析（LMqPCR HRMA），被修改用于快速分型粪肠球菌、金黄色葡萄球菌、肺炎克雷伯菌、鲍曼不动杆菌、铜绿假单胞菌和肠杆菌属（ESKAPE）病原体。与脉冲场凝胶电泳（PFGE）结果相比，该方法的一致性达到 97%（60/62 株分离株），这表明 LMqPCR HRMA 是一种快速准确的监测医院感染爆发的筛查工具。
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