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采用连接酶介导的实时 PCR 高分辨率熔解曲线分析方法快速进行 ESKAPE（屎肠球菌、金黄色葡萄球菌、肺炎克雷伯菌、鲍曼不动杆菌、铜绿假单胞菌和肠杆菌属）病原体的流行病学分型评估。

Evaluation of high-resolution melting curve analysis of ligation-mediated real-time PCR, a rapid method for epidemiological typing of ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter Species) pathogens.

机构信息

Department of Clinical Microbiology, Kalmar County Hospital, Kalmar, Sweden Department of Medicine and Optometry, Linnaeus University, Kalmar, Sweden

Department of Clinical Microbiology and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.

出版信息

J Clin Microbiol. 2014 Dec;52(12):4339-42. doi: 10.1128/JCM.02537-14. Epub 2014 Sep 17.

DOI:10.1128/JCM.02537-14
PMID:25232168
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4313309/
Abstract

A single-tube method, ligation-mediated real-time PCR high-resolution melt analysis (LMqPCR HRMA), was modified for the rapid typing of Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp. (ESKAPE) pathogens. A 97% agreement (60/62 isolates) was achieved in comparison to pulsed-field gel electrophoresis (PFGE) results, which indicates that LMqPCR HRMA is a rapid and accurate screening tool for monitoring nosocomial outbreaks.

摘要

一种单管方法，连接介导的实时 PCR 高分辨率熔解分析（LMqPCR HRMA），被修改用于快速分型粪肠球菌、金黄色葡萄球菌、肺炎克雷伯菌、鲍曼不动杆菌、铜绿假单胞菌和肠杆菌属（ESKAPE）病原体。与脉冲场凝胶电泳（PFGE）结果相比，该方法的一致性达到 97%（60/62 株分离株），这表明 LMqPCR HRMA 是一种快速准确的监测医院感染爆发的筛查工具。

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1
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Infect Drug Resist. 2013 Oct 8;6:115-23. doi: 10.2147/IDR.S35710.
2
Rapid bacterial whole-genome sequencing to enhance diagnostic and public health microbiology.快速的细菌全基因组测序以增强诊断和公共卫生微生物学。
JAMA Intern Med. 2013 Aug 12;173(15):1397-404. doi: 10.1001/jamainternmed.2013.7734.
3
Rapid bacterial genome sequencing: methods and applications in clinical microbiology.快速细菌基因组测序：在临床微生物学中的方法和应用。
Clin Microbiol Infect. 2013 Sep;19(9):803-13. doi: 10.1111/1469-0691.12217. Epub 2013 Apr 18.
4
Overview of molecular typing methods for outbreak detection and epidemiological surveillance.暴发检测和流行病学监测的分子分型方法概述。
Euro Surveill. 2013 Jan 24;18(4):20380. doi: 10.2807/ese.18.04.20380-en.
5
The future of antibiotics and resistance.抗生素与耐药性的未来。
N Engl J Med. 2013 Jan 24;368(4):299-302. doi: 10.1056/NEJMp1215093.
6
Routine use of microbial whole genome sequencing in diagnostic and public health microbiology.微生物全基因组测序在诊断和公共卫生微生物学中的常规应用。
PLoS Pathog. 2012;8(8):e1002824. doi: 10.1371/journal.ppat.1002824. Epub 2012 Aug 2.
7
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J Antimicrob Chemother. 2012 Oct;67(10):2307-8. doi: 10.1093/jac/dks247. Epub 2012 Jun 22.
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