β-肌动蛋白表达的细胞类型特异性及其在胃腺癌中的临床病理相关性
Cell-type specificity of β-actin expression and its clinicopathological correlation in gastric adenocarcinoma.
作者信息
Khan Shafqat A, Tyagi Monica, Sharma Ajit K, Barreto Savio G, Sirohi Bhawna, Ramadwar Mukta, Shrikhande Shailesh V, Gupta Sanjay
机构信息
Shafqat A Khan, Monica Tyagi, Ajit K Sharma, Sanjay Gupta, Epigenetics and Chromatin Biology Group, Cancer Research Institute, Advanced Centre for Treatment Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai, MH 410210, India.
出版信息
World J Gastroenterol. 2014 Sep 14;20(34):12202-11. doi: 10.3748/wjg.v20.i34.12202.
AIM
To investigate cell type specific distribution of β-actin expression in gastric adenocarcinoma and its correlation with clinicopathological parameters.
METHODS
β-actin is a housekeeping gene, frequently used as loading control, but, differentially expresses in cancer. In gastric cancer, an overall increased expression of β-actin has been reported using tissue disruptive techniques. At present, no histological data is available to indicate its cell type-specific expression and distribution pattern. In the present study, we analyzed β-actin expression and distribution in paired normal and tumor tissue samples of gastric adenocarcinoma patients using immunohistochemistry (IHC), a tissue non-disruptive technique as well as tissue disruptive techniques like reverse transcriptase-polymerase chain reaction (RT-PCR) and western blotting. Correlation of β-actin level with clinicopathological parameters was done using univariate analysis.
RESULTS
The results of this study showed significant overexpression, at both mRNA and protein level in tumor tissues as confirmed by RT-PCR (1.47 ± 0.13 vs 2.36 ± 0.16; P < 0.001) and western blotting (1.92 ± 0.26 vs 2.88 ± 0.32; P < 0.01). IHC revealed that β-actin expression is majorly distributed between epithelial and inflammatory cells of the tissues. Inflammatory cells showed a significantly higher expression compared to epithelial cells in normal (2.46 ± 0.13 vs 5.92 ± 0.23, P < 0.001), as well as, in tumor tissues (2.79 ± 0.24 vs 6.71 ± 0.14, P < 0.001). Further, comparison of immunostaining between normal and tumor tissues revealed that both epithelial and inflammatory cells overexpress β-actin in tumor tissues, however, significant difference was observed only in inflammatory cells (5.92 ± 0.23 vs 6.71 ± 0.14, P < 0.01). Moreover, combined expression in epithelial and inflammatory cells also showed significant increase (4.19 ± 0.15 vs 4.75 ± 0.14, P < 0.05) in tumor tissues. In addition, univariate analysis showed a positive correlation of β-actin level of inflammatory cells with tumor grade (P < 0.05) while epithelial cells exhibited negative correlation (P > 0.05).
CONCLUSION
In gastric cancer, β-actin showed an overall higher expression predominantly contributed by inflammatory or tumor infiltrating immune cells of the tissue microenvironment and correlates with tumor grade.
目的
研究β-肌动蛋白在胃腺癌中的细胞类型特异性分布及其与临床病理参数的相关性。
方法
β-肌动蛋白是一种管家基因，常被用作上样对照，但在癌症中表达存在差异。在胃癌中，使用组织破坏技术已报道β-肌动蛋白的总体表达增加。目前，尚无组织学数据表明其细胞类型特异性表达和分布模式。在本研究中，我们使用免疫组织化学（IHC）这一组织非破坏技术以及逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹等组织破坏技术，分析胃腺癌患者配对的正常和肿瘤组织样本中β-肌动蛋白的表达和分布。使用单因素分析评估β-肌动蛋白水平与临床病理参数的相关性。
结果
本研究结果显示，RT-PCR（1.47±0.13对2.36±0.16；P<0.001）和蛋白质免疫印迹（1.92±0.26对2.88±0.32；P<0.01）证实肿瘤组织中β-肌动蛋白在mRNA和蛋白质水平均显著过表达。免疫组织化学显示，β-肌动蛋白表达主要分布在组织的上皮细胞和炎症细胞之间。在正常组织（2.46±0.13对5.92±0.23，P<0.001）以及肿瘤组织（2.79±0.24对6.71±0.14，P<0.001）中，炎症细胞的表达明显高于上皮细胞。此外，正常组织和肿瘤组织免疫染色的比较显示，肿瘤组织中的上皮细胞和炎症细胞均过表达β-肌动蛋白，然而，仅在炎症细胞中观察到显著差异（5.92±0.23对6.71±0.14，P<0.01）。此外，肿瘤组织中上皮细胞和炎症细胞的联合表达也显著增加（4.19±0.15对4.75±0.14，P<0.05）。另外，单因素分析显示炎症细胞的β-肌动蛋白水平与肿瘤分级呈正相关（P<0.05），而上皮细胞呈负相关（P>0.05）。
结论
在胃癌中，β-肌动蛋白总体表达较高，主要由组织微环境中的炎症或肿瘤浸润免疫细胞所致，且与肿瘤分级相关。
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