Carini C, Margolick J, Yodoi J, Ishizaka K
Johns Hopkins University School of Medicine, Baltimore, Md.
Int Arch Allergy Appl Immunol. 1989;88(1-2):116-8. doi: 10.1159/000234760.
Peripheral blood mononuclear cells of human immunodeficiency virus infected patients were cultured in the presence or absence of 10 micrograms/ml human IgE, and culture filtrates were fractionated on IgE-Sepharose. IgE-binding factors were assessed in the acid eluate fraction from IgE-Sepharose by both rosette inhibition assay and radioimmunoassay. Among 22 cases studied, mononuclear cells from 9 patients formed IgE-binding factors in the absence of IgE, and those of 13 cases formed the factors upon incubation with IgE. The major cell source of IgE-binding factors was T cells. In 2 cases of human immunodeficiency virus infection, Fc epsilon receptors were detected on both Leu-2+ and Leu-3+ T cells.
在有或没有10微克/毫升人IgE存在的情况下，培养人类免疫缺陷病毒感染患者的外周血单核细胞，并将培养滤液在IgE-琼脂糖凝胶上进行分级分离。通过玫瑰花结抑制试验和放射免疫测定法评估来自IgE-琼脂糖凝胶的酸性洗脱级分中的IgE结合因子。在研究的22例病例中，9例患者的单核细胞在没有IgE的情况下形成IgE结合因子，13例患者的单核细胞在与IgE孵育后形成这些因子。IgE结合因子的主要细胞来源是T细胞。在2例人类免疫缺陷病毒感染病例中，在Leu-2 +和Leu-3 + T细胞上均检测到Fcε受体。
