Carini C, Margolick J, Yodoi J, Ishizaka K
Subdepartment of Immunology, School of Medicine, Johns Hopkins University, Baltimore, MD.
Proc Natl Acad Sci U S A. 1988 Dec;85(23):9214-8. doi: 10.1073/pnas.85.23.9214.
Peripheral blood mononuclear cells of 10 out of 26 patients with human immunodeficiency virus type 1 (HIV-1) released IgE-binding factors, as determined by two independent assays. The formation of the factors by the mononuclear cells was enhanced by incubation of the cells with homologous IgE. In the presence of IgE, peripheral blood mononuclear cells from 15 of the 26 patients formed a detectable amount of IgE-binding factors, whereas those of normal individuals of allergic rhinitis patients failed to do so. The major source of IgE-binding factors was the T cells of the HIV-1-infected patients. The CD8+ T cells from a HIV-1-infected patient formed IgE-binding factors upon incubation with IgE, and type II receptors for Fc epsilon were detected on both CD4+ T cells and CD8+ T cells in one of five patients studied. It was also found that culture supernatants of mononuclear cells from HIV-1-infected patients released soluble factors that induce normal human T cells to form IgE-binding factors. The results suggest that lymphocytes of some HIV-1-infected patients are activated to produce lymphokines regulating formation of IgE-binding factors.
通过两项独立检测发现，26例1型人类免疫缺陷病毒（HIV-1）患者中有10例的外周血单个核细胞释放IgE结合因子。单核细胞与同源IgE孵育可增强因子的形成。在存在IgE的情况下，26例患者中有15例的外周血单个核细胞形成了可检测量的IgE结合因子，而过敏性鼻炎患者的正常个体的外周血单个核细胞则未形成。IgE结合因子的主要来源是HIV-1感染患者的T细胞。一名HIV-1感染患者的CD8 + T细胞与IgE孵育后形成IgE结合因子，在研究的五名患者中的一名患者的CD4 + T细胞和CD8 + T细胞上均检测到FcεII型受体。还发现，HIV-1感染患者的单核细胞培养上清液释放出可诱导正常人T细胞形成IgE结合因子的可溶性因子。结果表明，一些HIV-1感染患者的淋巴细胞被激活以产生调节IgE结合因子形成的淋巴因子。
