Tanasienko Iryna V, Yemets Alla I, Finiuk Nataliya S, Stoika Rostyslav R, Blume Yaroslav B
Department of Genomics and Molecular Biotechnology, Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Osipovskogo St. 2a, Kyiv, 04123, Ukraine.
Cell Biol Int. 2015 Mar;39(3):243-5. doi: 10.1002/cbin.10381. Epub 2014 Oct 6.
Different transformation systems and vectors have been improved to increase the effectiveness of transformation and achieve stable expression of target genes. Because classical direct and indirect transformation processes commonly suffer from instability of a gene in the environment, gene deletion, transgene silencing, and poor gene transfer efficiency. Nowadays, gene transformation technologies are based on the use of new carriers (nanoparticles, carbon nanotubes, whiskers, and polymers) characterized by better efficiency and reproducibility for the direct DNA delivery into cells. In this review, we have focused on the novel DMAEM-based direct DNA delivery system and its possible applications for cell transformation.
不同的转化系统和载体已得到改进，以提高转化效率并实现靶基因的稳定表达。因为传统的直接和间接转化过程通常存在基因在环境中不稳定、基因缺失、转基因沉默以及基因转移效率低等问题。如今，基因转化技术基于使用新型载体（纳米颗粒、碳纳米管、晶须和聚合物），这些载体具有更高的效率和可重复性，可将DNA直接递送至细胞中。在本综述中，我们重点关注了基于DMAEM的新型直接DNA递送系统及其在细胞转化中的潜在应用。
