Institute of Pharmaceutics, Zhejiang University, 388 Yuhangtang Road, Hangzhou 310058, Zhejiang, PR China.
Int J Pharm. 2010 Feb 15;386(1-2):232-42. doi: 10.1016/j.ijpharm.2009.11.006. Epub 2009 Nov 14.
An increasing number of non-viral vectors are being developed for the use of gene delivery nowadays, among which cationic polymers and lipoplexes receive most attention. Most of these researches are focused on how to increase the transfection efficiency of non-viral vectors as well as the reduction of toxicity. In this review, we go over new strategies to reduce the toxicity of cationic polyplexes such as poly(ethylene-imine) and the construction of highly effective gene transfer vector lipoplexes. In addition, since transformation of gene expression system from two-dimensional (2D) substrate to 3D scaffold triggers far better transfection efficiency, the non-viral vectors applied in 3D transfection system have also been reviewed.
目前，越来越多的非病毒载体被开发用于基因传递，其中阳离子聚合物和脂质体受到了最多的关注。这些研究大多集中在如何提高非病毒载体的转染效率以及降低毒性上。在本文综述中，我们讨论了降低阳离子聚电解质（如聚乙烯亚胺）和高效基因转移载体脂质体毒性的新策略。此外，由于从二维（2D）基质到 3D 支架的基因表达系统的转化会引发更好的转染效率，因此我们还综述了用于 3D 转染系统的非病毒载体。
