DeCoster M A, DeVries G H
Dept. of Biochemistry and Molecular Biophysics, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
J Neurosci Res. 1989 Mar;22(3):283-8. doi: 10.1002/jnr.490220308.
Treatment of axolemma with pH 9 buffer results in a pellet enriched two-fold in the mitogen for cultured Schwann cells. Heparitinase treatment releases 8% of the mitogen into solution, while heparin selectively solubilizes the mitogen, resulting in an extract which has a specific mitogenic activity approximately 2.5 times greater than the mitogenicity of the starting axolemma membrane. These data support a model in which the axolemmal mitogen is a positively charged molecule associated with negatively charged sulfated proteoglycans.
用pH 9缓冲液处理轴膜，会得到一种沉淀，其中培养雪旺细胞的促细胞分裂剂富集了两倍。肝素酶处理会使8%的促细胞分裂剂释放到溶液中，而肝素则选择性地溶解促细胞分裂剂，从而得到一种提取物，其特定的促有丝分裂活性比起始轴膜的促有丝分裂活性大约高2.5倍。这些数据支持了一种模型，即轴膜促细胞分裂剂是一种与带负电荷的硫酸化蛋白聚糖相关的带正电荷的分子。
