转化生长因子-β1和-β2是大鼠雪旺氏细胞的促细胞分裂剂。
Transforming growth factors-beta 1 and beta 2 are mitogens for rat Schwann cells.
作者信息
Ridley A J, Davis J B, Stroobant P, Land H
机构信息
Imperial Cancer Research Fund, London, United Kingdom.
出版信息
J Cell Biol. 1989 Dec;109(6 Pt 2):3419-24. doi: 10.1083/jcb.109.6.3419.
Transforming growth factor-beta 1 (TGF-beta 1) and TGF-beta 2 were found to be potent mitogens for purified rat Schwann cells, each stimulating DNA synthesis in quiescent cells and also increasing their proliferation rate. Half-maximal stimulation of DNA synthesis occurred at approximately 0.1 ng/ml TGF-beta 1 or TGF-beta 2. Mitogenic stimulation by TGF-beta 1 and TGF-beta 2 was enhanced by forskolin, which activates adenylate cyclase, at concentrations up to 0.5 microM forskolin. However, at 5 microM forskolin, the synergistic interaction between forskolin and TGF-beta 1 was abolished. These results are in contrast to the observed synergy between forskolin and another Schwann cell mitogen, glial growth factor (GGF). Both 0.5 and 5 microM forskolin were found to enhance the stimulation of DNA synthesis by partially purified GGF (GGF-CM). As well as being functionally distinct, TGF-beta 1 and GGF-CM activities were also physically separable by chromatography on a Superose 12 gel permeation column. Thus, TGF-beta 1 and beta 2 are rat Schwann cell mitogens, and Schwann cells are one of the few normal cell populations to respond mitogenically to TGF-beta.
转化生长因子-β1（TGF-β1）和TGF-β2被发现是纯化大鼠雪旺细胞的有效促分裂原，它们均可刺激静止细胞中的DNA合成并提高其增殖速率。TGF-β1或TGF-β2对DNA合成的半数最大刺激浓度约为0.1 ng/ml。在浓度高达0.5 μM的福司可林（可激活腺苷酸环化酶）存在时，TGF-β1和TGF-β2的促有丝分裂刺激作用增强。然而，在5 μM福司可林时，福司可林与TGF-β1之间的协同相互作用消失。这些结果与观察到的福司可林与另一种雪旺细胞促分裂原神经胶质生长因子（GGF）之间的协同作用形成对比。已发现0.5 μM和5 μM的福司可林均可增强部分纯化的GGF（GGF-CM）对DNA合成的刺激作用。除了功能不同外，TGF-β1和GGF-CM的活性在Superose 12凝胶渗透柱上进行色谱分离时也可在物理上分开。因此，TGF-β1和β2是大鼠雪旺细胞促分裂原，并且雪旺细胞是少数对TGF-β有促有丝分裂反应的正常细胞群体之一。
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