Solarczyk Piotr, Majewska Anna C, Słodkowicz-Kowalska Anna
Department of Biology and Medical Parasitology, Medical Faculty I, Poznan University of Medical Sciences, 10 Fredry Street, 61-701, Poznań, Poland,
Acta Parasitol. 2014 Oct;59(4):763-6. doi: 10.2478/s11686-014-0279-2. Epub 2014 Sep 20.
Giardia duodenalis is an ubiquitous flagellate that infects humans and many species of animals. This species exhibits great biotypic and genetic diversity. In the present study, we established short- and long-term in vitro cultures of G. duodenalis trophozoites originating from red deer and Thomson's gazelle (artiodactyls) and genetically characterised the isolates by their glutamate dehydrogenase and triose phosphate isomerase gene sequences. The G. duodenalis isolates from red deer and the gazelle represented assemblages A (AIII sub-assemblage) and B. In conclusion, G. duodenalis assemblages and sub-assemblages can be associated with differences in growth rate in vitro cultures.
十二指肠贾第虫是一种广泛存在的鞭毛虫，可感染人类和许多动物物种。该物种表现出极大的生物型和遗传多样性。在本研究中，我们建立了源自马鹿和汤氏瞪羚（偶蹄目动物）的十二指肠贾第虫滋养体的短期和长期体外培养物，并通过其谷氨酸脱氢酶和磷酸丙糖异构酶基因序列对分离株进行了基因特征分析。来自马鹿和瞪羚的十二指肠贾第虫分离株分别代表A群（AIII亚群）和B群。总之，十二指肠贾第虫的群和亚群可能与体外培养物中的生长速率差异有关。
