Solarczyk Piotr, Majewska Anna C, Moskwa Bozena, Cabaj Władysław, Dabert Miroslawa, Nowosad Piotr
Department of Biology and Medical Parasitology, Poznań University of Medical Science, Faculty of Medicine I, 10 Fredry Street, 61-701 Poznań, Poland.
Folia Parasitol (Praha). 2012 Sep;59(3):237-40. doi: 10.14411/fp.2012.032.
A total of 181 faecal samples were collected from wild cervids in two regions of Poland. Giardia cysts were detected in one faecal specimen from red deer and in two samples from roe deer. Fragments of the beta-giardin (bg) triose phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes were successfully amplified from the Giardia isolate obtained from red deer, whereas only amplicons of bg and gdh were obtained from Giardia isolates derived from two roe deer. The result of genotyping and phylogenetic analysis showed that the G. duodenalis isolate from red deer belonged to sub-assemblage AIII, which has never been identified in humans, whereas isolates from roe deer clustered within zoonotic sub-assemblage AI. Further studies are necessary to explain which Giardia assemblages and/or sub-assemblages occur in wild cervids in various regions of the world. Moreover, the impact of Giardia infection on the health of wild cervids should also be elucidated.
从波兰两个地区的野生鹿类中总共采集了181份粪便样本。在一份马鹿粪便标本和两份狍粪便样本中检测到贾第虫囊肿。成功从马鹿分离出的贾第虫中扩增出β-贾第虫（bg）磷酸丙糖异构酶（tpi）和谷氨酸脱氢酶（gdh）基因片段，而从两份狍分离出的贾第虫中仅获得了bg和gdh的扩增子。基因分型和系统发育分析结果表明，来自马鹿的十二指肠贾第虫分离株属于AIII亚群，在人类中从未发现过，而来自狍的分离株聚集在人畜共患的AI亚群中。有必要进行进一步研究，以解释世界不同地区的野生鹿类中存在哪些贾第虫组合和/或亚组合。此外，还应阐明贾第虫感染对野生鹿类健康的影响。
