Körner T, Rath F W
Department of Internal Medicine, Martin Luther University Halle-Wittenberg, GDR.
Acta Histochem. 1989;85(1):73-7. doi: 10.1016/S0065-1281(89)80101-1.
Human liver specimens obtained by needle biopsy were embedded into glycolmethacrylate and enzyme histochemical investigations were carried out on semithin sections. By this method, we could demonstrate an activity of acid phosphatase (EC 3.1.3.2) by azo-coupling as well as by metal precipitation methods, of alkaline phosphatase (EC 3.1.3.1) by metal precipitation method, and of ATPases (EC 3.6.1.3 and EC 3.6.1.4) in ITO's cells. Special aspects of the application of enzyme histochemical methods to resin-embedded liver tissue are explained.
通过针吸活检获取的人类肝脏标本被包埋于甲基丙烯酸乙二醇酯中，并在半薄切片上进行酶组织化学研究。通过这种方法，我们能够用偶氮偶联法以及金属沉淀法证明酸性磷酸酶（EC 3.1.3.2）的活性，用金属沉淀法证明碱性磷酸酶（EC 3.1.3.1）的活性，以及在伊藤细胞中证明ATP酶（EC 3.6.1.3和EC 3.6.1.4）的活性。文中解释了酶组织化学方法应用于树脂包埋肝脏组织的一些特殊方面。
