Yoshioka T, Tanaka O
Department of Anatomy, Shimane Medical University, Izumo, Japan.
Int J Dev Neurosci. 1989;7(2):181-93. doi: 10.1016/0736-5748(89)90068-3.
In order to investigate the membrane activities underlying development of neural cells, a histochemical localization of Ca2+-ATPase, Mg2+-ATPase and alkaline phosphatase (AlPase) activities in the rat cerebellar cortex during postnatal development was carried out. In the developing cerebellar cortex, ATPase activity was mainly associated with the plasma membranes of Purkinje and granular cells. This activity appeared in the immature Purkinje cells at birth and was proportionally increased throughout postnatal development. It was observed that the ATPase activity of migratory granular cells during a critical period from 3 and 15 postnatal days was increased in a funicular pattern in the developing cerebellar cortex. Conversely, peak AlPase activity in the developing cerebellar cortex was localized in the proliferative external granular cells until 7 postnatal days. Apparently, these phosphatase activities were not present in Bergmann glial fibers during the course of granular cell migration. The present findings were taken to indicate that neuronal cells in the cerebellar cortex have acquired a membrane-bound ATPase which can participate in Ca2+ transport or ATP metabolism during the course of early postnatal development.
为了研究神经细胞发育过程中的膜活性，对出生后发育过程中大鼠小脑皮质中的Ca2 + -ATP酶、Mg2 + -ATP酶和碱性磷酸酶（AlPase）活性进行了组织化学定位。在发育中的小脑皮质中，ATP酶活性主要与浦肯野细胞和颗粒细胞的质膜相关。这种活性在出生时出现在未成熟的浦肯野细胞中，并在出生后的整个发育过程中按比例增加。据观察，在出生后3至15天的关键时期，迁移颗粒细胞的ATP酶活性在发育中的小脑皮质中呈索状模式增加。相反，发育中的小脑皮质中的AlPase活性峰值在出生后7天之前定位于增殖的外颗粒细胞中。显然，在颗粒细胞迁移过程中，伯格曼胶质纤维中不存在这些磷酸酶活性。目前的研究结果表明，小脑皮质中的神经元细胞在出生后早期发育过程中获得了一种膜结合的ATP酶，其可以参与Ca2 +转运或ATP代谢。
