Department of Plant Sciences, University of Hyderabad, Hyderabad, India.
Plant Biotechnol J. 2015 Jan;13(1):73-84. doi: 10.1111/pbi.12237. Epub 2014 Sep 18.
We have identified a transcript derived fragment (TDF) corresponding to SGT1 in a study of differential gene expression on the resistant wild peanut, Arachis diogoi, upon challenge from the late leaf spot pathogen, Phaeoisariopsis personata, and cloned its full-length cDNA followed by subsequent validation through q-PCR. Sodium nitroprusside, salicylic acid, ethephon and methyl jasmonate induced the expression of AdSGT1, while the treatment with abscisic acid did not elicit its up-regulation. AdSGT1 is localized to both nucleus and cytoplasm. Its overexpression induced hypersensitive-like cell death in tobacco under transient conditional expression using the estradiol system, and this conditional expression of AdSGT1 was also associated with the up-regulation of NtHSR203J, HMGR and HIN1, which have been shown to be associated with hypersensitive response in tobacco in earlier studies. Expression of the cDNA in a susceptible cultivated peanut variety enhanced its resistance against the late leaf spot pathogen, Phaeoisariopsis personata, while the heterologous expression in tobacco enhanced its resistance against Phytophthora parasitica var. nicotianae, Alternaria alternata var. nicotianae and Rhizoctonia solani. Constitutive expression in peanut was associated with the co-expression of resistance-related genes, CC-NB-LRR and some protein kinases.
我们在研究抗晚疫病野生花生（Arachis diogoi）的差异基因表达时，发现了一个与 SGT1 对应的转录本衍生片段（TDF），并克隆了其全长 cDNA，随后通过 q-PCR 进行了验证。亚硝基钠、水杨酸、乙烯利和茉莉酸甲酯诱导 AdSGT1 的表达，而脱落酸处理则没有上调其表达。AdSGT1 定位于细胞核和细胞质。在使用雌二醇系统进行瞬时条件表达时，其过表达在烟草中诱导类似于过敏反应的细胞死亡，并且这种 AdSGT1 的条件表达也与 NtHSR203J、HMGR 和 HIN1 的上调有关，在早期的研究中，这些基因与烟草中的过敏反应有关。在易感栽培花生品种中表达 cDNA 可增强其对晚疫病病原菌（Phaeoisariopsis personata）的抗性，而在烟草中的异源表达可增强其对寄生疫霉（Phytophthora parasitica var. nicotianae）、Alternaria alternata var. nicotianae 和立枯丝核菌（Rhizoctonia solani）的抗性。花生中的组成型表达与抗性相关基因 CC-NB-LRR 和一些蛋白激酶的共表达有关。
